Tyrosine Hydroxylase anticorps (pSer40)
Aperçu rapide pour Tyrosine Hydroxylase anticorps (pSer40) (ABIN337188)
Antigène
Voir toutes Tyrosine Hydroxylase (TH) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- pSer40
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Specificité
- Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectivity over dephospho TH.
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Purification
- Immunoaffinity purified
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Immunogène
- Phosphopeptide corresponding to amino acid residues surrounding the phosphoSer40 of rat tyrosine hydroxylase.
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Indications d'application
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Approved: IF (1:1000), IHC, IHC-Fr (1:1000), IHC-P (5 μg/mL), WB (1:1000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. -
Commentaires
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Target Species of Antibody: Rat
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol.
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Stock
- -20 °C,-80 °C
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Stockage commentaire
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Short term: -20°C
Long term: -80°C.
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- Tyrosine Hydroxylase (TH)
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Autre désignation
- TH / Tyrosine Hydroxylase
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Sujet
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Name/Gene ID: TH
Family: Monooxygenase
Synonyms: TH, DYT14, Dystonia 14, TYH, Tyrosine 3-hydroxylase, Tyrosine hydroxylase, DYT5b, Tyrosine 3-monooxygenase -
ID gène
- 7054
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UniProt
- P07101
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Pathways
- Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour
Antigène
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