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Tyrosine Hydroxylase anticorps (pSer40)

Cet anticorps anti-Tyrosine Hydroxylase Polyclonal Lapin (ABIN337188) détecte spécifiquement Tyrosine Hydroxylase dans WB, IHC, IF, IHC (p) et IHC (fro). L’anticorps est réactif avec des échantillons de Humain et Rat.
N° du produit ABIN337188
610,95 €
Plus frais de livraison 40,00 € et TVA
50 μL
Destination: France
Envoi sous 15 à 20 jours ouvrables

Aperçu rapide pour Tyrosine Hydroxylase anticorps (pSer40) (ABIN337188)

Antigène

Voir toutes Tyrosine Hydroxylase (TH) Anticorps
Tyrosine Hydroxylase (TH)

Reactivité

  • 146
  • 140
  • 116
  • 11
  • 9
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  • 5
  • 4
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Humain, Rat

Hôte

  • 142
  • 36
  • 6
  • 5
  • 2
Lapin

Clonalité

  • 125
  • 66
Polyclonal

Conjugué

  • 118
  • 10
  • 6
  • 6
  • 6
  • 6
  • 6
  • 6
  • 2
  • 2
  • 2
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Cet anticorp Tyrosine Hydroxylase est non-conjugé

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Épitope

    • 20
    • 15
    • 14
    • 12
    • 11
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
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    pSer40

    Specificité

    Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectivity over dephospho TH.

    Purification

    Immunoaffinity purified

    Immunogène

    Phosphopeptide corresponding to amino acid residues surrounding the phosphoSer40 of rat tyrosine hydroxylase.
  • Indications d'application

    Approved: IF (1:1000), IHC, IHC-Fr (1:1000), IHC-P (5 μg/mL), WB (1:1000)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

    Commentaires

    Target Species of Antibody: Rat

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol.

    Stock

    -20 °C,-80 °C

    Stockage commentaire

    Short term: -20°C
    Long term: -80°C.
  • Antigène

    Tyrosine Hydroxylase (TH)

    Autre désignation

    TH / Tyrosine Hydroxylase

    Sujet

    Name/Gene ID: TH
    Family: Monooxygenase

    Synonyms: TH, DYT14, Dystonia 14, TYH, Tyrosine 3-hydroxylase, Tyrosine hydroxylase, DYT5b, Tyrosine 3-monooxygenase

    ID gène

    7054

    UniProt

    P07101

    Pathways

    Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour
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