GFP anticorps
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- Antigène Voir toutes GFP Anticorps
- GFP (Green Fluorescent Protein (GFP))
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Reactivité
- Toutes les espèces
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp GFP est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC)
- Specificité
- Specific for EGFP/GFP/Venus
- Immunogène
- Synthetic peptides (a pentamer) from EGFP/GFP/Venus conjugated to an immunogenic carrier protein was used as the antigen.
- Isotype
- IgG
- Top Product
- Discover our top product GFP Anticorps primaire
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- Indications d'application
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A working concentration of 5-50 µg/ml is recommended.
The optimal concentration should be determined by the end user. - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
- Conseil sur la manipulation
- Avoid freeze and thaw cycles.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- GFP (Green Fluorescent Protein (GFP))
- Abstract
- GFP Produits
- Synonymes
- anticorps green fluorescent protein, anticorps gfp
- Classe de substances
- Viral Protein
- Sujet
- GFP is a naturally fluorescent protein, discovered by chance in the 1960s by Shimomura et al. GFP makes green light out of aequorin’s blue light. The chromophore is a modified Ser-Tyr-Gly sequence. GFP can emit light once this short tripeptide has adopted a cyclic conformation with the help of a oxygen. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488, Emission max (nm): 509, Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Also known as: (Enhanced) Green Fluorescence Protein.
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