Souris anti-Humain IgE (Fc Region) Anticorps

Détails pour le produit réf. ABIN363704, Fournisseur: Connectez-vous pour afficher
Antigène
Épitope
Fc Region
135
60
53
7
1
1
1
1
1
Reactivité
Humain
484
141
46
44
6
6
3
2
2
2
1
1
1
Hôte
Souris
291
286
67
52
10
6
3
1
1
Clonalité (Clone)
Monoclonal ()
Application
Functional Studies (Func), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
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Immunogène purified human myeloma IgE
Clone BSW17-NBS01
Isotype IgG1 kappa
Specificité Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes
Attributs du produit IGHE, chromosome 14q32.33
Purification Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B
Pureté > 95 %, tested by analytical Gel Permeation Chromatography
Sujet The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.
Domaine de recherche Immunology, Secondary Antibodies
Indications d'application When used for functional cellular assays (mast cell / basophil activation) at dilutions < 1:100, the Na-azide free formulation is recommended. Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb BSW17), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS, pH=7.2, 0.1 % NaN3
Agent conservateur Sodium azide
Précaution d'utilisation WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Conseil sur la manipulation Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
Stock 4 °C
Supplier Images
 image for Souris anti-Humain IgE (Fc Region) Anticorps (ABIN363704) product purity: gel permeation chromatography (Superose 12/HR)
 image for Souris anti-Humain IgE (Fc Region) Anticorps (ABIN363704) Target binding kinetics (Biacore
Produit citée dans: Rudolf, Zuercher, Nechansky, Ruf, Vogel, Miescher, Stadler, Kricek: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, 2000 (PubMed).

Background publications Rudolf, Furukawa, Miescher, Vogel, Kricek, Stadler: "Effect of anti-IgE antibodies on Fc epsilonRI-bound IgE." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 12, pp. 5646-52, 1997 (PubMed).

Czech, Stadler, Schuopf, Kapp: "IgE autoantibodies in atopic dermatitis--occurrence of different antibodies against the CH3 and the CH4 epitopes of IgE." dans: Allergy, Vol. 50, Issue 3, pp. 243-8, 1995 (PubMed).

Knutti-Mueller, Stadler, Magnusson, de Weck: "Human IgE synthesis in vitro. Detection with monoclonal antibodies." dans: Allergy, Vol. 41, Issue 6, pp. 457-67, 1987 (PubMed).