DDX5 anticorps (C-Term)

N° du produit ABIN374414

Détail du produit anti-DDX5 anticorps

Antigène: DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))

Épitope: C-Term

Reactivité: Humain, Souris, Rat, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp DDX5 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Séquence: C-PMIGYPMPTG YSQ

Specificité: This antibody detects DEAD Box Protein 5 at C-term.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide from the C-Terminus of the protein sequence according to NP_004387.1. Genename: DDX5

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 64000. Western blot: 0.11-0.3 μg/mL. Approx 70 kDa band observed in human epidermoidcarcinoma A431 lysates (calculated MW of 69.0 kDa according to NP_004387). Immunofluorescence: A customer has reported successful staining at a dilution of 1: 100 onHT-1080 cells with a Rhodamine conjugated secondary.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Détails sur DDX5

Antigène: DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))

Autre désignation: DDX5 (DDX5 Produits)

Synonymes: anticorps G17P1, anticorps HLR1, anticorps HUMP68, anticorps p68, anticorps 2600009A06Rik, anticorps Hlr1, anticorps wu:fa56a07, anticorps wu:fb11e01, anticorps wu:fb16c10, anticorps wu:fb53b05, anticorps ddx5, anticorps DEAD-box helicase 5, anticorps DEAD (Asp-Glu-Ala-Asp) box polypeptide 5, anticorps DEAD (Asp-Glu-Ala-Asp) box helicase 5, anticorps DEAD-box helicase 5 L homeolog, anticorps DDX5, anticorps Ddx5, anticorps ddx5, anticorps ddx5.L

Sujet: DDX belongs to the family of DEAD box proteins, characterized by the conserved motif Asp/Glu/Ala/Asp, which are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.Synonyms: DDX-5, DEAD box protein 5, G17P1, HELR, HLR1, Probable ATP-dependent RNA helicase DDX5, RNA helicase p68

Poids moléculaire: 69.1 kDa according to NP_004387

ID gène: 1655

NCBI Accession: NP_004387

UniProt: P17844

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Regulation of Muscle Cell Differentiation, Positive Regulation of Response to DNA Damage Stimulus