GFAP anticorps

N° du produit ABIN967500

Détail du produit anti-GFAP anticorps

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivité: Humain, Rat, Souris, Porc, Boeuf (Vache), Poulet, Lapin, Cobaye, Mouton, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GFAP est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marque: BD Pharmingen™

Réactivité croisée: Humain, Souris, Boeuf (Vache), Mouton, Chien, Porc, Lapin, Cobaye, Poulet, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Cow spinal cord homogenate

Clone: 1B4

Isotype: IgG2b

Information d'application

Indications d'application: Additional GFAP specific antibodies: clone 2E1 (ABIN967501), 4A11 (ABIN967499), and clones 1B4, 4A11, 2E1 combined and available as a cocktail” (ABIN967502). Applications include indirect immunofluorescence of tissue-cultured cells, immunohistochemical staining of formalin-fixed paraffin-embedded brain tissue sections (25 µg/ml), and western blot analysis (1-2 µg/ml). Rat brain is suggested as a positive control

Commentaires:

Related Products: ABIN967501, ABIN967499, ABIN967502

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-GFAP (ABIN967500)

McLendon, Bigner: "Immunohistochemistry of the glial fibrillary acidic protein: basic and applied considerations." dans: Brain pathology (Zurich, Switzerland), Vol. 4, Issue 3, pp. 221-8, (1994) (PubMed).

McLendon, Burger, Pegram, Eng, Bigner: "The immunohistochemical application of three anti-GFAP monoclonal antibodies to formalin-fixed, paraffin-embedded, normal and neoplastic brain tissues." dans: Journal of neuropathology and experimental neurology, Vol. 45, Issue 6, pp. 692-703, (1986) (PubMed).

Pegram, Eng, Wikstrand, McComb, Lee, Bigner: "Monoclonal antibodies reactive with epitopes restricted to glial fibrillary acidic proteins of several species." dans: Neurochemical pathology, Vol. 3, Issue 2, pp. 119-38, (1985) (PubMed).

Détails sur GFAP

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Autre désignation: GFAP (GFAP Produits)

Synonymes: anticorps GFAP, anticorps AI836096, anticorps cb345, anticorps etID36982.3, anticorps gfapl, anticorps wu:fb34h11, anticorps wu:fk42c12, anticorps xx:af506734, anticorps zgc:110485, anticorps glial fibrillary acidic protein, anticorps GFAP, anticorps LOC100136168, anticorps gfap, anticorps Gfap

Sujet: GFAP (Glial Fibrillary Acid Protein) is the major protein of glial filaments in differentiated astrocytes. We offer a panel ofnmonoclonal antibodies (4A11, 1B4, 2E1) that specifically recognize GFAP. They do not cross-react with other intermediate filaments such as vimentin, neurofilament proteins, desmin, keratin, neurotubules or microfilaments. Bovine spinal cord homogenate was used as immunogen. This antibody has broad species reactivity, recognizing GFAP in brain homogenates from human, mouse, rat, cow, sheep, dog, pig, rabbit, guinea pig and chicken. 1B4 is particularly useful for identifying GFAP in immunohistochemistry of frozen and formalin-fixed, paraffin-embedded brain tissue sections. Additional applications include western blot analysis and indirect immunofluorescence of tissue-cultured cells.
The antibody is routinely tested by immunohistochemical staining of formalin-fixed, paraffin-embedded brain tissue samples, which were subjected to pretreatment with citrate or tissue unmasking fluid (see figure).

Poids moléculaire: 50 kDa