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Rat IgG2a isotype control (Biotin)

FACS, IsoC IgG2a Biotin
N° du produit ABIN2688784
  • Antigène Tous les produits IgG2a
    IgG2a
    Hôte
    • 183
    • 74
    • 3
    • 1
    Rat
    Conjugué
    Biotin
    Application
    Flow Cytometry (FACS), Isotype Control (IsoC)
    Attributs du produit
    The R35-95 hybridoma was generated by hybridization of Y3 myeloma cells with spleen cells from LOU rats immunized with mouse immunoglobulins. The R35-95 hybridoma produces rat IgG2a, κ immunoglobulin that has no measurable reactivity with mouse immunoglobulins. The R35-95 immunoglobulin was selected as an isotype control following screening for low background binding on a variety of mouse and human tissues. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA)
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Mouse Pooled Immunoglobulin
    Isotype
    IgG2a
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Volume d'échantillon
    20 μL
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Stockage commentaire
    The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.
  • Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).

  • Antigène
    IgG2a
    Abstract
    IgG2a Produits
    Synonymes
    gamma-2a immunoglobulin heavy chain Isotype Control, IgG-2a Isotype Control
    Classe de substances
    Antibody
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