Emilin1 Kit ELISA

N° du produit ABIN5653808

Détail du produit Emilin1 Kit ELISA

Antigène: Emilin1 (EMILIN1) (Elastin Microfibril Interfacer 1 (EMILIN1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Elastin Microfibril Interface Located Protein 1 (EMILIN1). No significant cross-reactivity or interference between Elastin Microfibril Interface Located Protein 1 (EMILIN1) and analogues was observed.

Sensibilité: 0.56 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastin Microfibril Interface Located Protein 1 (EMILIN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Elastin Microfibril Interface Located Protein 1 (EMILIN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elastin Microfibril Interface Located Protein 1 (EMILIN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Elastin Microfibril Interface Located Protein 1 (EMILIN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Elastin Microfibril Interface Located Protein 1 (EMILIN1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Elastin Microfibril Interface Located Protein 1 (EMILIN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Emilin1 (EMILIN1) (Elastin Microfibril Interfacer 1 (EMILIN1))

Autre désignation: Elastin Microfibril Interface Located Protein 1 (EMILIN1 Produits)

Synonymes: im:7159005 Kit ELISA, si:dkey-20n10.2 Kit ELISA, wu:fc16f07 Kit ELISA, zgc:136949 Kit ELISA, EMILIN1 Kit ELISA, 5830419M17Rik Kit ELISA, AW229038 Kit ELISA, BB105748 Kit ELISA, EMILIN-1 Kit ELISA, gp115 Kit ELISA, EMI Kit ELISA, EMILIN Kit ELISA, elastin microfibril interfacer 1 Kit ELISA, elastin microfibril interfacer 1a Kit ELISA, elastin microfibril interfacer 1b Kit ELISA, elastin microfibril interfacer 1 L homeolog Kit ELISA, Emilin1 Kit ELISA, emilin1a Kit ELISA, emilin1b Kit ELISA, EMILIN1 Kit ELISA, emilin1.L Kit ELISA

Sujet:

Gene Name: Elastin Microfibril Interface Located Protein 1

Gene Aliases: EMI, gp115, Elastin Microfibril Interfacer 1

ID gène: 11117

UniProt: Q9Y6C2