EEF1G Kit ELISA

N° du produit ABIN5654004

Détail du produit EEF1G Kit ELISA

Antigène: EEF1G (Eukaryotic Translation Elongation Factor 1 gamma (EEF1G))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g). No significant cross-reactivity or interference between Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g) and analogues was observed.

Sensibilité: 12.4 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: EEF1G (Eukaryotic Translation Elongation Factor 1 gamma (EEF1G))

Autre désignation: Eukaryotic Translation Elongation Factor 1 Gamma (EEF1G Produits)

Synonymes: EF1G Kit ELISA, GIG35 Kit ELISA, eEF-1 gamma Kit ELISA, db03h10 Kit ELISA, fk76a09 Kit ELISA, mg:db03h10 Kit ELISA, wu:fk76a09 Kit ELISA, 2610301D06Rik Kit ELISA, AA407312 Kit ELISA, eef1g Kit ELISA, ef1g Kit ELISA, gig35 Kit ELISA, eukaryotic translation elongation factor 1 gamma Kit ELISA, eukaryotic translation elongation factor 1 gamma L homeolog Kit ELISA, eukaryotic translation elongation factor 1 gamma S homeolog Kit ELISA, EEF1G Kit ELISA, eef1g Kit ELISA, Eef1g Kit ELISA, eef1g.L Kit ELISA, eef1g.S Kit ELISA, EHI_119540 Kit ELISA, CMU_014590 Kit ELISA

Sujet:

Gene Name: Eukaryotic Translation Elongation Factor 1 Gamma

Gene Aliases: EF1G, GIG35

ID gène: 1937

UniProt: P26641