RASSF1 Kit ELISA

N° du produit ABIN5658354

Détail du produit RASSF1 Kit ELISA

Antigène: RASSF1 (Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Ras Association Domain Containing Protein 1 (RASSF1). No significant cross-reactivity or interference between Ras Association Domain Containing Protein 1 (RASSF1) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ras Association Domain Containing Protein 1 (RASSF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ras Association Domain Containing Protein 1 (RASSF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ras Association Domain Containing Protein 1 (RASSF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ras Association Domain Containing Protein 1 (RASSF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ras Association Domain Containing Protein 1 (RASSF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ras Association Domain Containing Protein 1 (RASSF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: RASSF1 (Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1))

Autre désignation: Ras Association Domain Containing Protein 1 (RASSF1 Produits)

Synonymes: RASSF1 Kit ELISA, zgc:92505 Kit ELISA, wu:fc17f02 Kit ELISA, zgc:112224 Kit ELISA, 123F2 Kit ELISA, NORE2A Kit ELISA, RASSF1A Kit ELISA, RDA32 Kit ELISA, REH3P21 Kit ELISA, AA536941 Kit ELISA, AU044980 Kit ELISA, Rassf1A Kit ELISA, Rassf1B Kit ELISA, Rassf1C Kit ELISA, NPR2-like, GATOR1 complex subunit Kit ELISA, Ras association (RalGDS/AF-6) domain family 1 Kit ELISA, Ras association domain family member 1 Kit ELISA, putative rassf1 Kit ELISA, Ras association domain family member 1 L homeolog Kit ELISA, Ras association (RalGDS/AF-6) domain family member 1 Kit ELISA, NPRL2 Kit ELISA, rassf1 Kit ELISA, RASSF1 Kit ELISA, Smp_157340 Kit ELISA, rassf1.L Kit ELISA, Rassf1 Kit ELISA

Sujet:

Gene Name: Ras Association Domain Containing Protein 1

Gene Aliases: 123F2, NORE2A, RASSF1A, RDA32, REH3P21