Humain Transportin 1 ELISA Kit

Recommended Transportin 1 Product (fourni par: Connectez-vous pour afficher )

Antigène
Transportin 1 (TNPO1) Kits ELISA
  • TNPO1
  • ATTRN1
  • F12A24.13
  • F12A24_13
  • TRANSPORTIN 1
  • transportin 1
  • DKFZp469O204
  • mip1
  • zgc:100858
  • JC310
  • MIP1
  • SIN1
  • SIN1b
  • SIN1g
  • IPO2
  • KPNB2
  • MIP
  • TRN
  • AU021749
  • D13Ertd688e
  • Kpnb2
  • Mip1
  • Znf667
  • transportin 1
  • major intrinsic protein of lens fiber a
  • mitogen-activated protein kinase associated protein 1
  • zinc finger protein 667
  • TNPO1
  • tnpo1
  • TRN1
  • mipa
  • MAPKAP1
  • Tnpo1
  • Zfp667
Reactivité
Humain
Alternatives
Kits with alternative reactivity to:
7
6
2
2
2
2
2
1
1
1
1
Type de méthode
Sandwich ELISA
Gamme de detection
3.12-200 ng/mL
Seuil minimal de détection
3.12 ng/mL
Application
ELISA
Fournisseur
Connectez-vous pour afficher
Supplier Product No.
Connectez-vous pour afficher
Request

Recevoir ce produit gratuitement

Envoyez-nous votre proposition de validation. J'aimerais valider ce produit

Savoir plus

Images

N° du produit ABIN1572842
875,63 €
Plus frais de livraison 40,00 € et TVA
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Fournisseur References Details
12.499185 ABIN1117590 Sandwich ELISA Serum, Plasma, Biological Fluids 3.125-200 ng/mL 3.125 ng/mL Connectez-vous pour afficher
1 ABIN511054 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 5.0-100 ng/mL 5.0 ng/mL Connectez-vous pour afficher
1 ABIN5525993 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 3.125-200 ng/mL 3.125 ng/mL Connectez-vous pour afficher
1 ABIN4973513 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 3.125-200 ng/mL 3.125 ng/mL Connectez-vous pour afficher
1 ABIN2952025 3.125-200 ng/mL 3.125 ng/mL Connectez-vous pour afficher
1 ABIN5071969 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Connectez-vous pour afficher

General

Antigène Transportin 1 (TNPO1) Kits ELISA
Reactivité Humain
Kits with alternative reactivity to:
(7), (6), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Type de méthode Sandwich ELISA
Gamme de detection 3.12-200 ng/mL
Seuil minimal de détection 3.12 ng/mL
Application ELISA
Fournisseur Connectez-vous pour afficher

Détail du produit Transportin 1 ELISA Kit

Détail du antigène Information d'application Stockage Images
Fonction The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of TNPO1 in human serum, plasma, tissue homogenates and other biological fluids.
Type d'échantillon Biological Fluids, Plasma, Serum, Tissue Homogenate
Méthode de détection Colorimetric
Analytical Method Quantitative
Specificité This assay has high sensitivity and excellent specificity for detection of this index.
Réactivité croisée (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensibilité 1.06 ng/mL
Ingrédients
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Matériel non inclus
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.

Détail du antigène

Détail du produit Transportin 1 ELISA Kit Information d'application Stockage Images Haut de la page
Antigène
Autre désignation TNPO1 (TNPO1 ELISA Kit Extrait)
ID gène 3842
UniProt Q92973
Pathways Signalisation PI3K-Akt, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Cellular Glucan Metabolic Process, Protein targeting to Nucleus, CXCR4-mediated Signaling Events

Information d'application

Détail du produit Transportin 1 ELISA Kit Détail du antigène Stockage Images Haut de la page
Volume d'échantillon 100 μL
Durée du test 1 - 4.5 h
Plaque Pre-coated
Protocole 1. Prepare all reagents, samples and standards
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 µL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 µL Stop Solution. Read at 450nm immediately.
Procédure de l'essai The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Précision du teste
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV&lt10%
  • Inter-assay: CV&lt12%
Restrictions For Research Use only

Stockage

Détail du produit Transportin 1 ELISA Kit Détail du antigène Information d'application Images Haut de la page
Précaution d'utilisation The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Conseil sur la manipulation The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Stock 4 °C,-20 °C
Date de péremption 12 months

Images

Détail du produit Transportin 1 ELISA Kit Détail du antigène Information d'application Stockage Haut de la page
Supplier Images
ELISA image for Transportin 1 (TNPO1) ELISA Kit (ABIN1572842) Transportin 1 (TNPO1) ELISA Kit