CD34 anticorps (FITC)

N° du produit ABIN1027678

Détail du produit anti-CD34 anticorps (FITC)

Antigène: CD34

Reactivité: Humain, Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD34 est conjugé à/à la FITC

Application: Flow Cytometry (FACS)

Specificité: The mouse monoclonal antibody 581 reacts with an extracellular epitope of CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells, it is gradually lost on progenitor cells. The antibody recognizes the class III CD34 epitope resistant to neuraminidase, chymopapain and glycoprotease.

Aucune reactivité croisée: Mouton

Réactivité croisée (Details): Human, Non-Human Primates

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Clone: 581

Isotype: IgG1 kappa

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Commentaires:

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Stockage

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.
Avoid prolonged exposure to light.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD34 (FITC) (ABIN1027678)

Sanz, Muñoz-A, Monserrat, Van-Den-Rym, Escoll, Ranz, Alvarez-Mon, de-la-Hera: "Ordering human CD34+CD10-CD19+ pre/pro-B-cell and CD19- common lymphoid progenitor stages in two pro-B-cell development pathways." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 13, pp. 5925-30, (2010) (PubMed).

Goardon, Nikolousis, Sternberg, Chu, Craddock, Richardson, Benson, Drayson, Standen, Vyas, Freeman: "Reduced CD38 expression on CD34+ cells as a diagnostic test in myelodysplastic syndromes." dans: Haematologica, Vol. 94, Issue 8, pp. 1160-3, (2009) (PubMed).

Ninos, Jefferies, Cogle, Kerr: "The thrombopoietin receptor, c-Mpl, is a selective surface marker for human hematopoietic stem cells." dans: Journal of translational medicine, Vol. 4, pp. 9, (2006) (PubMed).

Ono, Sharma, Smith, Burnett, Aurelian: "CD34+ cells in the peripheral blood transport herpes simplex virus DNA fragments to the skin of patients with erythema multiforme (HAEM)." dans: The Journal of investigative dermatology, Vol. 124, Issue 6, pp. 1215-24, (2005) (PubMed).

Kato, Ando, Nakamura, Muguruma, Sato, Yabe, Yabe, Hattori, Yasuda, Hotta: "Absence of a CD34- hematopoietic precursor population in recipients of CD34+ stem cell transplantation." dans: Bone marrow transplantation, Vol. 28, Issue 6, pp. 587-95, (2001) (PubMed).

Felschow, McVeigh, Hoehn, Civin, Fackler: "The adapter protein CrkL associates with CD34." dans: Blood, Vol. 97, Issue 12, pp. 3768-75, (2001) (PubMed).

Ando, Nakamura, Chargui, Matsuzawa, Tsuji, Kato, Hotta: "Extensive generation of human cord blood CD34(+) stem cells from Lin(-)CD34(-) cells in a long-term in vitro system." dans: Experimental hematology, Vol. 28, Issue 6, pp. 690-9, (2000) (PubMed).

Fluckiger, Sanz, Garcia-Lloret, Su, Hao, Kato, Quan, de la Hera, Crooks, Witte, Rawlings: "In vitro reconstitution of human B-cell ontogeny: from CD34(+) multipotent progenitors to Ig-secreting cells." dans: Blood, Vol. 92, Issue 12, pp. 4509-20, (1999) (PubMed).

Janowska-Wieczorek, Marquez, Nabholtz, Cabuhat, Montaño, Chang, Rozmus, Russell, Edwards, Turner: "Growth factors and cytokines upregulate gelatinase expression in bone marrow CD34(+) cells and their transmigration through reconstituted basement membrane." dans: Blood, Vol. 93, Issue 10, pp. 3379-90, (1999) (PubMed).

Détails sur CD34

Antigène: CD34

Autre désignation: CD34 (CD34 Produits)

Synonymes: anticorps AU040960, anticorps CD34 molecule, anticorps CD34 antigen, anticorps CD34, anticorps Cd34

Sujet: CD34 Molecule,CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.

ID gène: 947

UniProt: P28906