VCAM1 anticorps

N° du produit ABIN1042491

Cet anticorps anti-VCAM1 est un anticorps Souris Monoclonal détectant VCAM1 dans FACS, IP et IHC (fro). Adapté pour Humain. Ce Primary Antibody a été cité dans 5+ publications.

Aperçu rapide pour VCAM1 anticorps (ABIN1042491)

Antigène: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp VCAM1 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 4B9

Détail du produit anti-VCAM1 anticorps

Specificité: The antibody binds exclusively to the cytokine-induced endothelial cell adhesion protein, vascular cell adhesion molecule-1 (VCAM-1).

Attributs du produit: Tumour marker

Purification: Purified

Isotype: IgG1

Information d'application

Indications d'application: 4B9 is suitable for immunoblotting, immunoprecipitation, immunocytochemistry and immuno-histochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:25 – 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.

Commentaires:

4B9 is a mouse IgG1 monoclonal antibody generated by immunization of Balb/c mice with human umbilical vein endothelium (HUVE) that were non-enzymatically harvested after stimulation for 24h with recombinant human (rh) TNF-alpha (10 ng/ml). Splenocytes were fused with the NS-1 nonsecretory murine myeloma line.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.09% sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Références pour anticorps anti-VCAM1 (ABIN1042491)

Chuluyan, Osborn, Lobb, Issekutz: "Domains 1 and 4 of vascular cell adhesion molecule-1 (CD106) both support very late activation antigen-4 (CD49d/CD29)-dependent monocyte transendothelial migration." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 6, pp. 3135-4, (1995) (PubMed).

Pinola, Saksela, Tiisala, Renkonen: "Human NK cells expressing alpha 4 beta 1/beta 7 adhere to VCAM-1 without preactivation." dans: Scandinavian journal of immunology, Vol. 39, Issue 2, pp. 131-6, (1994) (PubMed).

Bacchi, Marsh, Perkins, Carithers, McVicar, Hudkins, Benjamin, Harlan, Lobb, Alpers: "Expression of vascular cell adhesion molecule (VCAM-1) in liver and pancreas allograft rejection." dans: The American journal of pathology, Vol. 142, Issue 2, pp. 579-91, (1993) (PubMed).

Ockenhouse, Tegoshi, Maeno, Benjamin, Ho, Kan, Thway, Win, Aikawa, Lobb: "Human vascular endothelial cell adhesion receptors for Plasmodium falciparum-infected erythrocytes: roles for endothelial leukocyte adhesion molecule 1 and vascular cell adhesion molecule 1." dans: The Journal of experimental medicine, Vol. 176, Issue 4, pp. 1183-9, (1992) (PubMed).

Carlos, Schwartz, Kovach, Yee, Rosa, Osborn, Chi-Rosso, Newman, Lobb, Rosso: "Vascular cell adhesion molecule-1 mediates lymphocyte adherence to cytokine-activated cultured human endothelial cells." dans: Blood, Vol. 76, Issue 5, pp. 965-70, (1990) (PubMed).

Détails sur VCAM1

Antigène: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Autre désignation: VCAM-1

Sujet: Vascular cell adhesion molecule-1 (VCAM-1) is induced on human umbilical vein endothelium (HUVE) by recombinant human tumor necrosis factor - alpha (rh TNF-alpha), rh interleukin-1 (IL-1) and lipopolysaccharide (LPS) and is involved in adherence of lymphocytic cell lines to HUVE. VCAM-1 is only minimally expressed on unstimulated endothelium. Molecular cloning and sequencing of VCAM-1 predicts a 69-kDa core protein with 6 potential N-linked glycosylation sites, if the protein is fully glycosylated a mature protein of about 90kDa is yielded. VCAM-1 mediates a component of the adherence of Peripheral Blood Lymphocytes (PBL) to rh TNF-stimulated HUVE, this CD18 independent mechanism of lymphocyte adherence to cytokine stimulated endothelium may be an important pathway of lymphocyte emigration at sites of inflammation and immune reaction.

Pathways: Carbohydrate Homeostasis