IDO1 anticorps

N° du produit ABIN1043733

N° du produit (Fournisseur): 200-301-e57

Cet anticorps anti-IDO1 est un anticorps Souris Monoclonal détectant IDO1 dans WB, ELISA, IHC, IP et FM. Adapté pour Humain et Souris. Ce Primary Antibody a été cité dans 4+ publications.

Aperçu rapide pour IDO1 anticorps (ABIN1043733)

Antigène: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IDO1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Clone: 10-1

Détail du produit anti-IDO1 anticorps

N° du produit (Fournisseur): 200-301-e57

Fournisseur: Rockland

Fonction: IDO1 Antibody

Réactivité croisée (Details): This antibody is specific for human and mouse IDO1 protein.

Attributs du produit: Synonyms: mouse anti-IDO1 antibody, Ido antibody, Indo, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase, Ido1, Ido-1

Purification: Anti-IDO1 was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Anti-IDO1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with fragment of recombinant human and mouse IDO1 protein followed by hybridoma development.

Immunogen Type: Recombinant Protein

Isotype: IgG3

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Anti-IDO1 antibody has been tested in ELISA, IP, and Western Blot. This antibody is suitable for use in IHC and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:500-1:1500

Immunoprecipitation Dilution: 10-100 μL

ELISA Dilution: 1:5000-1:50000

IF Microscopy Dilution: 1:50-1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-IDO1 (ABIN1043733)

Meyer, Klatte, Dinh, Harries, Reithmayer, Meyer, Sinclair, Paus: "Evidence that the bulge region is a site of relative immune privilege in human hair follicles." dans: The British journal of dermatology, Vol. 159, Issue 5, pp. 1077-85, (2009) (PubMed).

Batista, Juhász, Muzik, Kupsky, Barger, Chugani, Mittal, Sood, Chakraborty, Chugani: "Imaging correlates of differential expression of indoleamine 2,3-dioxygenase in human brain tumors." dans: Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, Vol. 11, Issue 6, pp. 460-6, (2009) (PubMed).

Zhu, Ji, Wang, Zhang, Liu, Zhang, Matsushima, Cao, Zhang: "Synovial autoreactive T cells in rheumatoid arthritis resist IDO-mediated inhibition." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 11, pp. 8226-33, (2007) (PubMed).

Mellor, Munn: "IDO expression by dendritic cells: tolerance and tryptophan catabolism." dans: Nature reviews. Immunology, Vol. 4, Issue 10, pp. 762-74, (2004) (PubMed).

Détails sur IDO1

Antigène: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Autre désignation: Ido1

Sujet: Background: Indoleamine 2, 3-dioxygenase1 (IDO1) is a 41-42 kD intracellular enzyme that catabolizes tryptophan into kynurenine. IDO1 modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO1 effects on immune suppression are due to decreased tryptophan availability and the generation of tryptophan metabolites, resulting in negative effects on T lymphocytes, including proliferation, function and survival. IDO1 may be involved in the suppression of the immune response to tumors, and blocking the IDO1 pathway may be a potential target for immuno and cancer therapy. IDO1 is expressed in a wide variety of tissues and can be upregulated by interferon gamma and other inflammatory cytokines.

ID gène: 3620

NCBI Accession: NP_002155

UniProt: P14902

Pathways: Activated T Cell Proliferation