IL-2 anticorps (Biotin)

N° du produit ABIN1043809

N° du produit (Fournisseur): 209-406-b95

Cet anticorps Lapin Polyclonal détecte spécifiquement IL-2 dans WB, ELISA et IHC. Il présente une réactivité envers Humain et a été mentionné dans 1 publication.

Aperçu rapide pour IL-2 anticorps (Biotin) (ABIN1043809)

Antigène: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-2 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-IL-2 anticorps (Biotin)

N° du produit (Fournisseur): 209-406-b95

Fournisseur: Rockland

Fonction: IL-2 Antibody Biotin Conjugated

Attributs du produit: Synonyms: rabbit anti-IL-2 biotin conjugated Antibody, rabbit anti-Interleukin-2 biotin conjugated antibody, IL-2, IL2, T-cell growth factor, TCGF, Aldesleukin, Lymphokine

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56 °C for 30 minutes.

Immunogène:

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-2 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1,000-1:5,000

Application Note: This purified antibody has been tested in western blotting and suitable in ELISA. By western blot a band approximately 15 kDa in size corresponding to human IL-2 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:2,000-1:10,000

ELISA Dilution: 1:20,000-1:100,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.1 mg/ml

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-IL-2 (Biotin) (ABIN1043809)

Bazan: "Unraveling the structure of IL-2." dans: Science (New York, N.Y.), Vol. 257, Issue 5068, pp. 410-3, (1992) (PubMed).

Détails sur IL-2

Antigène: IL-2 (IL2) (Interleukin 2 (IL2))

Autre désignation: IL2

Sujet: Background: IL-2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.

ID gène: 3558

NCBI Accession: NP_000577

UniProt: P60568

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation