GDF15 anticorps (Growth Differentiation Factor 15) (C-Term) (Biotin)

Details for Product anti-GDF15 Antibody No. ABIN1043920, Fournisseur: Connectez-vous pour afficher
Antigène
  • GDF15
  • GDF-15
  • MIC-1
  • MIC1
  • NAG-1
  • PDF
  • PLAB
  • PTGFB
  • SBF
  • growth differentiation factor 15
  • GDF15
  • Gdf15
Épitope
C-Term
54
41
17
15
13
11
10
8
8
6
5
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivité
Humain, Souris
280
84
46
4
4
3
3
Hôte
Lapin
229
54
20
6
Clonalité
Polyclonal
Conjugué
Cet anticorp GDF15 est conjugé à/à la Biotin
38
29
15
6
6
5
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
265
179
81
48
26
13
13
11
10
10
7
1
1
1
1
Options
Fournisseur
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N° du produit (Fournisseur)
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Immunogène Nag-1 Antibody Biotin Conjugated antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of human NAG-1 protein.  A residue of cysteine was added to facilitate coupling to KLH.
Immunogen Type: Peptide
Isotype IgG
Specificité Nag-1 Antibody Biotin Conjugated antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.  This antibody reacts with the C-terminus of endogenous NAG-1 protein from human and mouse tissues.  A BLAST analysis suggests reactivity with NAG-1 from chimpanzee and macaque based on a 100% homology. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence.  Cross-reactivity with NAG-1 from other sources has not been determined.
Attributs du produit Non-steroidal anti-inflammatory drug (NSAID) activated gene (NAG-1) is a member of the transforming growth factor-beta (TGF-beta) superfamily. NAG-1 is also known as Macrophage Inhibitory Cytokine-1 (MIC-1), Growth Differentiation Factor 15 (GDF15), Placental Bone Morphogenetic Protein (PLAB), or Prostate Derived Factor (PDF). NAG-1 is expressed in human placenta, prostate and colon. It possesses antitumorigenic and proapoptotic activities. NAG-1 expression is dramatically increased in inflammation, injury and malignancy. Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate. In a number of studies, NAG-1 expression was increased by a number of NSAIDs. This increase in expression may correlate with the chemopreventive effect NSAIDs seem to have with certain cancers. NAG-1 expression is also induced by PPAR gamma ligands and by several dietary compounds such as conjugated linoleic acids (CLAs), naturally occurring fatty acids in ruminant food products, indoles, epicatechin gallate, and genistein. Induced expression of NAG-1 results in stimulation of apoptosis and inhibition of cell growth. Inhibition of NAG-1 induced expression by small interference RNA (siRNA) results in repression of induced apoptosis. NAG-1 expression is regulated by a numbers of transcription factors such as ERG-1 and Sp1. EGR-1 may be necessary for NSAID-induced NAG-1 expression. The study of expression of NAG-1 proteins, including variants, is important to define their potential role as serum biomarkers for cancer diagnosis, treatment monitoring, epidemiology study, and nutrition surveys.
Plasmids, Primers & others Plasmids, Primers & others GDF15 products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation Nag-1 (GDF15 Antibody Extrait)
Sujet Non-steroidal anti-inflammatory drug (NSAID) activated gene (NAG-1) is a member of the transforming growth factor-beta (TGF-beta) superfamily. NAG-1 is also known as Macrophage Inhibitory Cytokine-1 (MIC-1), Growth Differentiation Factor 15 (GDF15), Placental Bone Morphogenetic Protein (PLAB), or Prostate Derived Factor (PDF). NAG-1 is expressed in human placenta, prostate and colon. It possesses antitumorigenic and proapoptotic activities. NAG-1 expression is dramatically increased in inflammation, injury and malignancy. Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate. In a number of studies, NAG-1 expression was increased by a number of NSAIDs. This increase in expression may correlate with the chemopreventive effect NSAIDs seem to have with certain cancers. NAG-1 expression is also induced by PPAR gamma ligands and by several dietary compounds such as conjugated linoleic acids (CLAs), naturally occurring fatty acids in ruminant food products, indoles, epicatechin gallate, and genistein. Induced expression of NAG-1 results in stimulation of apoptosis and inhibition of cell growth. Inhibition of NAG-1 induced expression by small interference RNA (siRNA) results in repression of induced apoptosis. NAG-1 expression is regulated by a numbers of transcription factors such as ERG-1 and Sp1. EGR-1 may be necessary for NSAID-induced NAG-1 expression. The study of expression of NAG-1 proteins, including variants, is important to define their potential role as serum biomarkers for cancer diagnosis, treatment monitoring, epidemiology study, and nutrition surveys.
Synonyms: NAG-1, GDF15, MIC-1, nonsteroidal anti-inflammatory drug-activated gene, NSAID-activated gene 1 protein, growth differentiation factor 15, macrophage inhibitory compound 1, prostate-derived factor
ID gène 9518
UniProt Q99988
Indications d'application Nag-1 Antibody Biotin Conjugated antibody is suitable for ELISA and western blotting of human and mouse NAG-1 protein.  For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 (N-terminal), H variant or D variant specific antibodies.  Specific conditions for reactivity should be optimized by the end user.  Expect bands in Western blots of approximately 14 and 28 kDa in size corresponding to NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract.
Commentaires

Gene Name: GDF15

Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C/-20 °C
Stockage commentaire Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Date de péremption 12 months
Images (Fournisseur)
 image for anti-Growth Differentiation Factor 15 (GDF15) (C-Term) antibody (Biotin) (ABIN1043920) anti-Growth Differentiation Factor 15 (GDF15) (C-Term) antibody (Biotin)
 image for anti-Growth Differentiation Factor 15 (GDF15) (C-Term) antibody (Biotin) (ABIN1043920) anti-Growth Differentiation Factor 15 (GDF15) (C-Term) antibody (Biotin) (Image 2)
Western Blotting (WB) image for anti-Growth Differentiation Factor 15 (GDF15) (C-Term) antibody (Biotin) (ABIN1043920) Western blot using affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows de...
Immunohistochemistry (IHC) image for anti-Growth Differentiation Factor 15 (GDF15) (C-Term) antibody (Biotin) (ABIN1043920) Immunohistochemistry of Rabbit anti NAG1 antibody (C-terminal specific) at 20X in col...
Immunohistochemistry (IHC) image for anti-Growth Differentiation Factor 15 (GDF15) (C-Term) antibody (Biotin) (ABIN1043920) Immunohistochemistry of Rabbit anti NAG1 antibody (C-terminal specific) at 20X in col...
Produit citée dans: Baek, Eling: "Changes in gene expression contribute to cancer prevention by COX inhibitors." dans: Progress in lipid research, Vol. 45, Issue 1, pp. 1-16, 2006 (PubMed).

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