Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

Nestin anticorps (AA 1484-1500)

NES Reactivité: Humain WB, IHC, ELISA Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN104564
  • Antigène Voir toutes Nestin (NES) Anticorps
    Nestin (NES)
    Épitope
    • 16
    • 16
    • 15
    • 10
    • 10
    • 8
    • 7
    • 7
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1484-1500
    Reactivité
    • 131
    • 69
    • 51
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 102
    • 39
    • 4
    • 2
    • 1
    Lapin
    Clonalité
    • 107
    • 41
    Polyclonal
    Conjugué
    • 65
    • 10
    • 10
    • 9
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    Cet anticorp Nestin est non-conjugé
    Application
    • 81
    • 50
    • 49
    • 47
    • 41
    • 40
    • 39
    • 29
    • 25
    • 15
    • 8
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA
    Attributs du produit
    Concentration Definition: by UV absorbance at 280 nm
    Immunogène
    This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1484-1500 of human Nestin protein.
    Immunogentype:Recombinant
    Isotype
    IgG
    Top Product
    Discover our top product NES Anticorps primaire
  • Indications d'application
    Affinity purified Anti-Nestin has been tested for use in ELISA, Immunohistochemistry (IHC) and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 200-220 kDa in size corresponding to Nestin protein by western blotting in the appropriate cell lysate or extract.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
  • Mokrý, Cízková, Filip, Ehrmann, Osterreicher, Kolár, English: "Nestin expression by newly formed human blood vessels." dans: Stem cells and development, Vol. 13, Issue 6, pp. 658-64, (2005) (PubMed).

    Aihara, Sugawara, Torii, Hosaka, Kurihara, Saito, Takeuchi: "Angiogenic endothelium-specific nestin expression is enhanced by the first intron of the nestin gene." dans: Laboratory investigation; a journal of technical methods and pathology, Vol. 84, Issue 12, pp. 1581-92, (2005) (PubMed).

    Yaworsky, Kappen: "Heterogeneity of neural progenitor cells revealed by enhancers in the nestin gene." dans: Developmental biology, Vol. 205, Issue 2, pp. 309-21, (1999) (PubMed).

    Dahlstrand, Zimmerman, McKay, Lendahl: "Characterization of the human nestin gene reveals a close evolutionary relationship to neurofilaments." dans: Journal of cell science, Vol. 103 ( Pt 2), Issue 1, pp. 589-97, (1993) (PubMed).

    Hockfield, McKay: "Identification of major cell classes in the developing mammalian nervous system." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 5, Issue 12, pp. 3310-28, (1986) (PubMed).

  • Antigène
    Nestin (NES)
    Autre désignation
    Nestin (NES Produits)
    Synonymes
    anticorps paranemin, anticorps NES, anticorps AA166324, anticorps C78523, anticorps ESTM46, anticorps nestin, anticorps nestin S homeolog, anticorps NES, anticorps nes.S, anticorps Nes, anticorps nes
    Sujet
    Nestin, is a large intermediate filament protein (class Type VI) that was first identified with a monoclonal antibody by Hockfield and McKay (1985).  Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube.  Nestin is expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells.  Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.  Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.
    Synonyms: nestin, ESTM 46 antibody, FLJ 21841 antibody, FLJ21841 antibody, Intermediate filament protein antibody
    ID gène
    10763, 38176300
    UniProt
    P48681
Vous êtes ici:
Support technique