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Nestin anticorps

Name: Nestin anticorps
Brand: antibodies-online
SKU: ABIN7161178
Price: 415 EUR
Availability: InStock

NES Reactivité: Humain WB, IHC, ELISA, FACS, IF Hôte: Souris Monoclonal 2C2F7 unconjugated

Western Blot Positive WB detected in: U251 whole cell lysate, SH-SY5Y whole cell lysate All lanes: NES antibody at 3 μg/mL Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 178 kDa Observed band size: 260 kDa

Overlay histogram showing Hela cells stained with ABIN7161178 (red line).

FACS

Immunohistochemistry of paraffin-embedded human tonsil tissue using ABIN7161178 at dilution of 1:100

IHC

Immunohistochemistry of paraffin-embedded human kidney tissue using ABIN7161178 at dilution of 1:100

IHC

Immunofluorescent analysis of Hela cells using ABIN7161178 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of PC-3 cells using ABIN7161178 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of U251 cells using ABIN7161178 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunohistochemistry of paraffin-embedded human melanoma using ABIN7161178 at dilution of 1:100

IHC

Overlay histogram showing U251 cells stained with ABIN7161178 (red line).

FACS

9 images

N° du produit ABIN7161178

Fiche de données

Antigène Voir toutes Nestin (NES) Anticorps

Nestin (NES)
Reactivité
129

69

51

4

3

2

2

2

1

1

1

1
Humain
Hôte
101

38

4

2

1
Souris
Anticorps secondaires appropriés
Clonalité
106

40
Monoclonal
Conjugué
65

10

9

8

7

5

4

4

4

4

4

4

3

3

3

3

3

1

1

1
Cet anticorp Nestin est non-conjugé
Application
79

48

48

45

40

39

39

29

25

15

8

6

2

2

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Réactivité croisée

Humain

Purification

Protein G purified

Immunogène

Recombinant Human Nestin protein

Clone

2C2F7

Isotype

IgG1, IgG1 kappa

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anti-Nestin (NES) (AA 419-588) antibody
NES Reactivité: Humain WB, ELISA, FACS Hôte: Souris Monoclonal 1A9G12 unconjugated

Indications d'application

Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:500, IF:1:50-1:200,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Agent conservateur

ProClin

Précaution d'utilisation

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

-20 °C,-80 °C

Stockage commentaire

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Antigène

Nestin (NES)

Autre désignation

NES (NES Produits)

Synonymes

anticorps paranemin, anticorps NES, anticorps AA166324, anticorps C78523, anticorps ESTM46, anticorps nestin, anticorps nestin S homeolog, anticorps NES, anticorps nes.S, anticorps Nes, anticorps nes

Sujet

Background: Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Aliases: Nestin, NES, Nbla00170

UniProt

P48681