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Bestrophin 4 anticorps (Internal Region)

BEST4 Reactivité: Humain, Singe IHC (p), IHC Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1048355
  • Antigène Voir toutes Bestrophin 4 (BEST4) Anticorps
    Bestrophin 4 (BEST4)
    Épitope
    • 15
    • 2
    • 2
    • 2
    Internal Region
    Reactivité
    • 28
    • 8
    • 7
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Singe
    Hôte
    • 28
    Lapin
    Clonalité
    • 28
    Polyclonal
    Conjugué
    • 7
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Bestrophin 4 est non-conjugé
    Application
    • 26
    • 13
    • 13
    • 7
    • 5
    • 3
    • 3
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
    Marque
    IHC-plus™
    Specificité
    Human BEST4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Rabbit (93%) Hamster (87%) Pig (80%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 15 amino acid peptide from internal region of human BEST4. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Rabbit (93%), Hamster (87%), Pig (80%).

    Type of Immunogen: Synthetic peptide
  • Indications d'application
    Approved: IHC, IHC-P (2.5 μg/mL)
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    Bestrophin 4 (BEST4)
    Autre désignation
    BEST4 (BEST4 Produits)
    Synonymes
    anticorps CG7259, anticorps CT22395, anticorps Dmel\\CG7259, anticorps dbest4, anticorps BEST4, anticorps VMD2L2, anticorps vmd2l2, anticorps Bestrophin 4, anticorps bestrophin 4, anticorps Best4, anticorps BEST4, anticorps best4
    Sujet
    Name/Gene ID: BEST4
    Subfamily: Bestrophin
    Family: Ion channel

    Synonyms: BEST4, Bestrophin-4, Bestrophin 4, VMD2L2
    ID gène
    266675
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