Dopamine d2 Receptor anticorps (Cytoplasmic Domain)

N° du produit ABIN1048504

Détail du produit anti-Dopamine d2 Receptor anticorps

Antigène: Dopamine d2 Receptor (DRD2) (Dopamine Receptor D2 (DRD2))

Épitope: Cytoplasmic Domain

Reactivité: Humain, Chien, Boeuf (Vache), Cheval, Lapin, Roussette (Chauve-souris)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Dopamine d2 Receptor est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human DRD2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Ferret, Bat, Bovine, Dog, Elephant, Panda, Horse, Rabbit (100%) Gibbon, Monkey, Pig (94%) Marmoset (81%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human DRD2. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Ferret, Bat, Bovine, Dog, Elephant, Panda, Horse, Rabbit (100%), Gibbon, Monkey, Pig (94%), Marmoset (81%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 - 20 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur Dopamine d2 Receptor

Antigène: Dopamine d2 Receptor (DRD2) (Dopamine Receptor D2 (DRD2))

Autre désignation: DRD2 / Dopamine Receptor D2 (DRD2 Produits)

Synonymes: anticorps D2DR, anticorps D2R, anticorps drd2b, anticorps d2r2, anticorps Drd-2, anticorps d2dr, anticorps d2r, anticorps drd2, anticorps drd2-B, anticorps DRD2, anticorps D2, anticorps CG18741, anticorps CG7569, anticorps DAMB, anticorps DDR2, anticorps DOPR2, anticorps DOPR99B, anticorps Dar2, anticorps Dmel\\CG18741, anticorps DopD1, anticorps DopR99B, anticorps DopRII, anticorps dopamine receptor D2, anticorps dopamine receptor D2 like, anticorps dopamine receptor D2 L homeolog, anticorps dopamine receptor D2 S homeolog, anticorps Dopamine 1-like receptor 2, anticorps DRD2, anticorps drd2l, anticorps drd2, anticorps Drd2, anticorps drd2.L, anticorps drd2.S, anticorps Dop1R2

Sujet: Name/Gene ID: DRD2
Subfamily: Dopamine
Family: GPCR

Synonyms: DRD2, D2 dopamine receptor, D2R, Dopamine receptor D2 isoform, Dopamine 2 receptor, D(2) dopamine receptor, D2DR, Dopamine D2 receptor, Dopamine receptor D2

ID gène: 1813

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity