Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

FZD10 anticorps (N-Term)

FZD10 Reactivité: Humain, Singe IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1048619
  • Antigène Voir toutes FZD10 Anticorps
    FZD10 (Frizzled Family Receptor 10 (FZD10))
    Épitope
    • 15
    • 6
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivité
    • 41
    • 37
    • 20
    • 7
    • 7
    • 7
    • 5
    • 5
    • 3
    • 2
    Humain, Singe
    Hôte
    • 45
    Lapin
    Clonalité
    • 44
    • 1
    Polyclonal
    Conjugué
    • 22
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp FZD10 est non-conjugé
    Application
    • 37
    • 16
    • 13
    • 13
    • 9
    • 6
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marque
    IHC-plus™
    Specificité
    Human FZD10 / Frizzled 10. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Monkey (100%) Gibbon, Hamster, Panda, Dog (93%) Marmoset, Mouse, Bovine, Horse, Pig (86%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 14 amino acid peptide from N-terminal extracellular domain of human FZD10 / Frizzled 10. Percent identity with other species by BLAST analysis: Human, Monkey (100%), Gibbon, Hamster, Panda, Dog (93%), Marmoset, Mouse, Bovine, Horse, Pig (86%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product FZD10 Anticorps primaire
  • Indications d'application
    Approved: IHC, IHC-P (6 - 16 μg/mL)
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    FZD10 (Frizzled Family Receptor 10 (FZD10))
    Autre désignation
    FZD10 / Frizzled 10 (FZD10 Produits)
    Synonymes
    anticorps CD350, anticorps FZ-10, anticorps Fz10, anticorps FzE7, anticorps hFz10, anticorps cFz-10, anticorps Fz-10, anticorps Xfr9, anticorps Xfz10, anticorps frizzled-10, anticorps frizzled10, anticorps fz10, anticorps fze7, anticorps hfz10, anticorps fk48e04, anticorps fz4, anticorps fzb, anticorps wu:fk48e04, anticorps zg04, anticorps Xfz10A, anticorps fzd10a, anticorps Xfz10B, anticorps Xfz9, anticorps fzd10b, anticorps fzd9, anticorps frizzled class receptor 10, anticorps frizzled class receptor 10 L homeolog, anticorps frizzled class receptor 10 S homeolog, anticorps FZD10, anticorps fzd10, anticorps Fzd10, anticorps fzd10.L, anticorps fzd10.S
    Sujet
    Name/Gene ID: FZD10
    Subfamily: Frizzled
    Family: GPCR

    Synonyms: FZD10, CD350 antigen, CD350, FZ-10, Frizzled homolog 10, HFz10, Frizzled 10, Frizzled family receptor 10, Frizzled-10, FzE7, Fz10
    ID gène
    11211
    Pathways
    Signalisation WNT
Vous êtes ici:
Support technique