FZD4 anticorps (N-Term)

N° du produit ABIN1048623

Détail du produit anti-FZD4 anticorps

Antigène: FZD4 (Frizzled Family Receptor 4 (FZD4))

Épitope: N-Term

Reactivité: Humain, Rat, Boeuf (Vache), Chien, Singe, Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FZD4 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human FZD4 / Frizzled 4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Rat, Dolphin, Hamster, Elephant, Panda, Dog, Bovine (100%) Mouse, Horse, Pig (94%) Rabbit (89%) Lizard (83%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 18 amino acid peptide from N-terminal extracellular domain of human FZD4 / Frizzled 4. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Rat, Dolphin, Hamster, Elephant, Panda, Dog, Bovine (100%), Mouse, Horse, Pig (94%), Rabbit (89%), Lizard (83%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (30 - 60 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur FZD4

Antigène: FZD4 (Frizzled Family Receptor 4 (FZD4))

Autre désignation: FZD4 / Frizzled 4 (FZD4 Produits)

Synonymes: anticorps CG4626, anticorps DFz4, anticorps Dfz4, anticorps Dm Fz4, anticorps Dmel\\CG4626, anticorps Fz4, anticorps anon-WO0170980.10, anticorps anon-WO0170980.11, anticorps fz1, anticorps zg01, anticorps CD344, anticorps EVR1, anticorps FEVR, anticorps FZD4S, anticorps Fz-4, anticorps FzE4, anticorps GPCR, anticorps hFz4, anticorps frizzled4, anticorps fz4, anticorps FZ-4, anticorps frizzled 4, anticorps frizzled class receptor 4, anticorps frizzled-4, anticorps frizzled class receptor 4 S homeolog, anticorps fz4, anticorps fzd4, anticorps Tsp_10376, anticorps FZD4, anticorps Fzd4, anticorps fzd4.S

Sujet: Name/Gene ID: FZD4
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD4, CD344, CD344 antigen, EVR1, Frizzled family receptor 4, Frizzled-4, Fz-4, Fz4, HFz4, Exudative vitreoretinopathy 1, FEVR, FZD4S, FzE4, Frizzled 4, Frizzled homolog 4, WNT receptor frizzled-4

ID gène: 8322

Pathways: Signalisation WNT, Hormone Transport, Sensory Perception of Sound