GLP2R anticorps (N-Term)

N° du produit ABIN1048667

L’anticorps Lapin Polyclonal anti-GLP2R a été validé pour WB, IHC, IHC (p), IP, IF et ICC. Il convient pour détecter GLP2R dans des échantillons de Humain, Singe et Cobaye.

Aperçu rapide pour GLP2R anticorps (N-Term) (ABIN1048667)

Antigène: GLP2R (Glucagon-Like Peptide 2 Receptor (GLP2R))

Reactivité: Humain, Singe, Cobaye

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GLP2R est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-GLP2R anticorps

Épitope: N-Term

Marque: IHC-plus™

Specificité: Human GLP2R. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%) Gibbon (94%) Bovine, Dog, Elephant, Panda, Pig (89%) Bat, Rabbit (83%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 18 amino acid peptide from N-terminal extracellular domain of human GLP2R. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%), Gibbon (94%), Bovine, Dog, Elephant, Panda, Pig (89%), Bat, Rabbit (83%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: ICC, IF, IHC, IHC-P (10 - 20 μg/mL), IP, WB

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur GLP2R

Antigène: GLP2R (Glucagon-Like Peptide 2 Receptor (GLP2R))

Autre désignation: GLP2R / Glp-2

Sujet: Name/Gene ID: GLP2R
Subfamily: Glucagon
Family: GPCR

Synonyms: GLP2R, GLP-2-R, GLP-2 receptor, GLP-2R, Glp2 receptor

ID gène: 9340

Pathways: Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, cAMP Metabolic Process, Feeding Behaviour