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PAK6 anticorps (Linker Domain)

PAK6 Reactivité: Humain, Cheval, Chien, Singe IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1049227
  • Antigène Voir toutes PAK6 Anticorps
    PAK6 (P21-Activated Kinase 6 (PAK6))
    Épitope
    • 15
    • 15
    • 8
    • 7
    • 7
    • 7
    • 6
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Linker Domain
    Reactivité
    • 69
    • 45
    • 10
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Humain, Cheval, Chien, Singe
    Hôte
    • 84
    Lapin
    Clonalité
    • 83
    Polyclonal
    Conjugué
    • 40
    • 6
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Cet anticorp PAK6 est non-conjugé
    Application
    • 63
    • 38
    • 26
    • 26
    • 18
    • 15
    • 15
    • 12
    • 6
    • 4
    • 3
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marque
    IHC-plus™
    Specificité
    Human PAK6. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Dog, Horse (100%) Goat, Elephant, Panda, Rabbit (94%) Hamster, Bovine (89%) Mouse, Bat (83%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 18 amino acid peptide from Linker domain of human PAK6. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Dog, Horse (100%), Goat, Elephant, Panda, Rabbit (94%), Hamster, Bovine (89%), Mouse, Bat (83%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product PAK6 Anticorps primaire
  • Indications d'application
    Approved: IHC, IHC-P (3.5 μg/mL)
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    PAK6 (P21-Activated Kinase 6 (PAK6))
    Autre désignation
    PAK6 (PAK6 Produits)
    Synonymes
    anticorps PAK5, anticorps 4732456M09, anticorps pak6, anticorps si:ch211-158b22.3, anticorps p21 (RAC1) activated kinase 6, anticorps p21 protein (Cdc42/Rac)-activated kinase 6, anticorps p21 protein (Cdc42/Rac)-activated kinase 6b, anticorps PAK6, anticorps Pak6, anticorps pak6b
    Sujet
    Name/Gene ID: PAK6
    Subfamily: PAK (STE20)
    Family: Protein Kinase

    Synonyms: PAK6, PAK5, p21-activated kinase 6, p21-activated protein kinase 6, PAK-5, PAK-6, p21(CDKN1A)-activated kinase 6
    ID gène
    56924
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