Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

RORC anticorps (Ligand Binding Domain)

RORC Reactivité: Humain, Singe IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1049299
  • Antigène Voir toutes RORC Anticorps
    RORC (RAR-Related Orphan Receptor C (RORC))
    Épitope
    • 16
    • 7
    • 7
    • 6
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ligand Binding Domain
    Reactivité
    • 96
    • 55
    • 23
    • 9
    • 8
    • 7
    • 6
    • 6
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Singe
    Hôte
    • 79
    • 17
    • 2
    • 1
    • 1
    • 1
    Lapin
    Clonalité
    • 82
    • 19
    Polyclonal
    Conjugué
    • 53
    • 7
    • 5
    • 5
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Cet anticorp RORC est non-conjugé
    Application
    • 81
    • 37
    • 24
    • 24
    • 14
    • 13
    • 10
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marque
    IHC-plus™
    Specificité
    Human ROR Gamma. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Aucune reactivité croisée
    Macaque rhésus
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%) Mouse, Hamster, Rabbit (94%) Rat, Elephant, Panda, Bat, Bovine, Horse (89%) Dog (83%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 18 amino acid peptide from ligand-binding domain of human ROR Gamma. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%), Mouse, Hamster, Rabbit (94%), Rat, Elephant, Panda, Bat, Bovine, Horse (89%), Dog (83%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product RORC Anticorps primaire
  • Indications d'application
    Approved: IHC, IHC-P (20 μg/mL)
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    RORC (RAR-Related Orphan Receptor C (RORC))
    Autre désignation
    RORC / ROR gamma (RORC Produits)
    Synonymes
    anticorps NR1F3, anticorps RORG, anticorps RZR-GAMMA, anticorps RZRG, anticorps TOR, anticorps Nr1f3, anticorps RORgamma, anticorps Thor, anticorps RAR related orphan receptor C, anticorps RAR-related orphan receptor C, anticorps RAR-related orphan receptor gamma, anticorps RORC, anticorps Rorc
    Sujet
    Name/Gene ID: RORC
    Subfamily: NR1 Thyroid hormone-like
    Family: NHR

    Synonyms: RORC, Nuclear receptor ROR-gamma, Nuclear receptor RZR-gamma, RAR-related orphan receptor C, RORG, RORgamma, TOR, ROR Gamma, RZRG, NR1F3, RZR-GAMMA
    ID gène
    6097
    UniProt
    P51449
    Pathways
    Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway
Vous êtes ici:
Support technique