SLC44A2 anticorps (Internal Region)

N° du produit ABIN1049339

Détail du produit anti-SLC44A2 anticorps

Antigène: SLC44A2 (Solute Carrier Family 44, Member 2 (SLC44A2))

Épitope: Internal Region

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SLC44A2 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human SLC44A2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla (100%) Gibbon, Monkey, Marmoset (94%) Orangutan, Galago, Dog (88%) Rat, Hamster, Elephant, Panda, Horse, Pig, Guinea pig (81%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 16 amino acid peptide from internal region of human SLC44A2. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Gibbon, Monkey, Marmoset (94%), Orangutan, Galago, Dog (88%), Rat, Hamster, Elephant, Panda, Horse, Pig, Guinea pig (81%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur SLC44A2

Antigène: SLC44A2 (Solute Carrier Family 44, Member 2 (SLC44A2))

Autre désignation: SLC44A2 / CTL2 (SLC44A2 Produits)

Synonymes: anticorps CTL2, anticorps PP1292, anticorps 1110028E10Rik, anticorps RGD1309680, anticorps slc44a2, anticorps ctl2, anticorps MGC78933, anticorps SLC44A2, anticorps DKFZp469A0714, anticorps fc26c03, anticorps wu:fc26c03, anticorps solute carrier family 44 member 2, anticorps solute carrier family 44, member 2, anticorps solute carrier family 44 (choline transporter), member 2 L homeolog, anticorps Choline transporter-like protein 2, anticorps solute carrier family 44 (choline transporter), member 2, anticorps SLC44A2, anticorps Slc44a2, anticorps slc44a2.L, anticorps ctl2, anticorps slc44a2, anticorps Corgl_0495

Sujet: Name/Gene ID: SLC44A2
Subfamily: Choline transporter-like
Family: Transporter

Synonyms: SLC44A2, CTL2, PP1292

ID gène: 57153

Pathways: SARS-CoV-2 Protein Interactome