PPARG anticorps (N-Term)

N° du produit ABIN105801

N° du produit (Fournisseur): 600-401-418

Cet anticorps anti-PPARG est un anticorps Lapin Polyclonal détectant PPARG dans WB et ELISA. Adapté pour Souris.

Aperçu rapide pour PPARG anticorps (N-Term) (ABIN105801)

Antigène: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PPARG est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-PPARG anticorps

Épitope: N-Term

N° du produit (Fournisseur): 600-401-418

Fournisseur: Rockland

Fonction: PPAR gamma 2 Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against human PPAR gamma isoform 2 protein.

Attributs du produit: Synonyms: rabbit anti-PPAR gamma 2 antibody, Peroxisome proliferator-activated receptor gamma, Nuclear receptor subfamily 1 group C member 3, NR1C3, PPARG, PPAR gamma, PPARG2 antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human PPAR gamma 2.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a single band approximately 55 kDa in size corresponding to PPARg2 by western blot in the appropriate tissue or cell lysate.

Western Blot Dilution: 1:1,000 - 1:3,000

ELISA Dilution: 1:4,000 - 1:40,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur PPARG

Antigène: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Autre désignation: PPARG

Sujet: Background: Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones.  Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.

ID gène: 5468, 20336229

UniProt: P37231

Pathways: Signalisation MAPK, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation