PKM anticorps

N° du produit ABIN106387

N° du produit (Fournisseur): 100-1178

Cet anticorps Chèvre Polyclonal détecte spécifiquement PKM dans ELISA, WB, IHC et IP. Il présente une réactivité envers Lapin et a été mentionné dans 1 publication.

Aperçu rapide pour PKM anticorps (ABIN106387)

Antigène: PKM (Pyruvate Kinase M1/2 (PKM))

Reactivité: Lapin

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PKM est non-conjugé

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Détail du produit anti-PKM anticorps

N° du produit (Fournisseur): 100-1178

Fournisseur: Rockland

Fonction: Pyruvate Kinase Antibody

Réactivité croisée (Details): This product has been reported to react with all forms of pyruvate kinase (pan M-PK).

Attributs du produit: Synonyms: goat anti-Pyruvate Kinase Antibody, Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase 2/3 antibody, Cytosolic thyroid hormone-binding protein antibody, PKM2

Purification: Anti-PYRUVATE KINASE Antibody immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Pyruvate Kinase [Rabbit Muscle].

Immunogène:

Immunogen: Pyruvate Kinase [Rabbit Muscle]

Immunogen Type: Native Protein

Information d'application

Indications d'application:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-PYRUVATE KINASE Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. Specific conditions should be optimized by user.

Western Blot Dilution: 1:5,000 - 1:20,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:50,000 - 1:200,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 40 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Références pour anticorps anti-PKM (ABIN106387)

Shimada, Shinagawa, Ishii: "Modulation of M2-type pyruvate kinase activity by the cytoplasmic PML tumor suppressor protein." dans: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 13, Issue 3, pp. 245-54, (2008) (PubMed).

Détails sur PKM

Antigène: PKM (Pyruvate Kinase M1/2 (PKM))

Autre désignation: PKM

Sujet: Background: Pyruvate kinase (EC: 2.7.1.40) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. This process also requires a magnesium ion. The enzyme is a 'transferase' under the international classification of enzymes. This step is the final one in the glycolytic pathway, which produces pyruvate molecules, the final product of aerobic glycolysis. However, in anaerobic glycolysis, lactate dehydrogenase will utilize the NADH produced by glyceraldehyde phosphate dehydrogenase to reduce pyruvate to lactate. In humans, there are two pyruvate kinase isozymes: type M (muscle, SwissProt P14618) and type L,R (liver and erythrocyte, SwissProt P30613). The isozymes differ in primary structure and regulation. This reaction has a large positive free energy change, one of three in glycolysis. All three such steps regulate the overall activity of the pathway, and are, in general, irreversible under wild-type conditions. Anti-Pyruvate kinase antibody is idea for investigators involved in cytokines and growth factor research.

ID gène: 100008676

NCBI Accession: NP_001182574

UniProt: P11974

Pathways: L'effet Warburg