Urease anticorps

N° du produit ABIN108075

Détail du produit anti-Urease anticorps

Antigène: Urease (URE)

Reactivité: Haricots-sabres

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Urease est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Réactivité croisée (Details): Cross reactivity against Urease from other tissues and species may occur but have not been specifically determined.

Pureté: Anti-UREASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Urease [Jack Bean]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-UREASE Antibody has been assayed against 1.0 µg of Urease [Jack Bean] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:15.000 to 1:60.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur Urease

Antigène: Urease (URE)

Autre désignation: Urease (URE Produits)

Synonymes: anticorps F12B7.10, anticorps F12B7_10, anticorps urease, anticorps urease, anticorps URE, anticorps CNH01900

Sujet: Synonyms: Urea Amidohydrolase antibody

UniProt: P07374