PABP anticorps

N° du produit ABIN108538

Cet anticorps Souris Monoclonal détecte spécifiquement PABP dans WB, ELISA, IF, IP, FACS et ICC. Il présente une réactivité envers Humain, Lapin, Poulet, Xenopus laevis et Hamster et a été mentionné dans 3+ publications.

Aperçu rapide pour PABP anticorps (ABIN108538)

Antigène: PABP (PABPC1) (Poly(A) Binding Protein, Cytoplasmic 1 (PABPC1))

Reactivité: Humain, Lapin, Poulet, Xenopus laevis, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PABP est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 10E10

Détail du produit anti-PABP anticorps

Purification: Protein G

Immunogène: Recombinant PABP (Human) expressed from its 1.85 kbp cDNA, NcoI to SspI

Isotype: IgG2b

Information d'application

Indications d'application: Optimal antibody dilution should be determined by titration, however try:WB: Use at an assay dependent dilution. Detects a band of approximately 70 kDa (predicted molecular weight: 71 kDa)ICC/IF: Use at an assay dependent dilution. PubMed: 17977970Flow Cyt: Use 1 μg for 106 cells

Commentaires:

Myeloma, fusion partners: SP2/0

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody (from supernatant) containing PBS + 0.1 % sodium azide,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Références pour anticorps anti-PABP (ABIN108538)

Gorgoni, Richardson, Burgess, Anderson, Wilkie, Gautier, Martins, Brook, Sheets, Gray: "Poly(A)-binding proteins are functionally distinct and have essential roles during vertebrate development." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 19, pp. 7844-9, (2011) (PubMed).

Unsworth, Raguz, Edwards, Higgins, Yagüe: "mRNA escape from stress granule sequestration is dictated by localization to the endoplasmic reticulum." dans: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 9, pp. 3370-80, (2010) (PubMed).

Görlach, Burd, Dreyfuss: "The mRNA poly(A)-binding protein: localization, abundance, and RNA-binding specificity." dans: Experimental cell research, Vol. 211, Issue 2, pp. 400-7, (1994) (PubMed).

Détails sur PABP

Antigène: PABP (PABPC1) (Poly(A) Binding Protein, Cytoplasmic 1 (PABPC1))

Autre désignation: PABP

Sujet: Synonyms:
PAB 1 antibody,PAB1 antibody,PABP 1 antibody,PABP1 antibody,PABPC 1 antibody,PABPC1antibody,PABPC2 antibody,PABPL1 antibody,Poly A binding protein 1 antibody,Poly A binding protein cytoplasmic1 antibody,Polyadenylate binding protein 1 antibody

ID gène: 117282

OMIM: 604679

UniProt: P22626

Pathways: SARS-CoV-2 Protein Interactome