CD40 Ligand anticorps

N° du produit ABIN1100607

Cet anticorps anti-CD40 Ligand est un anticorps Chèvre Polyclonal détectant CD40 Ligand dans WB, IHC, ELISA et IHC (p). Adapté pour Humain.

Aperçu rapide pour CD40 Ligand anticorps (ABIN1100607)

Antigène: CD40 Ligand (CD40LG)

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp CD40 Ligand est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-CD40 Ligand anticorps

Marque: IHC-plus™

Specificité: Human sCD40 Ligand

Purification: Immunoaffinity purified

Immunogène: E.coli derived recombinant Human sCD40 Ligand.

Type of Immunogen: Cell extract

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7421 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. ELISA: To detect hsCD40-Ligand by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sCD40-Ligand (LS-C104498) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsCD40-Ligand. Western Blot: To detect hsCD40-Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40-Ligand is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur CD40 Ligand

Antigène: CD40 Ligand (CD40LG)

Autre désignation: CD40LG / CD40L / CD154

Sujet: Name/Gene ID: CD40LG
Family: TNF

Synonyms: CD40LG, CD154, CD154 antigen, CD40-L, CD40 ligand, CD40L, gp39, HIGM1, IGM, IMD3, T-BAM, TNFSF5, CD40 antigen ligand, CD54, HCD40L, T-B cell-activating molecule, T-cell antigen Gp39, TNF-related activation protein

ID gène: 959

UniProt: P29965

Pathways: Signalisation NF-kappaB, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints