HA-Tag anticorps (C-Term)

N° du produit ABIN1101941

L’anticorps anti-HA-Tag Polyclonal Lapin est utilisé pour la détection de HA-Tag. Il a été validé pour WB, ELISA, IP, IHC, IHC (p) et ChIP.

Aperçu rapide pour HA-Tag anticorps (C-Term) (ABIN1101941)

Antigène: HA-Tag

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HA-Tag est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP)

Détail du produit anti-HA-Tag anticorps

Épitope: C-Term

Séquence: YPYDVPDYA

Specificité: This affinity purified Anti-HA antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. To date, this antibody has reacted with all HA-tagged proteins tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.

Purification: Affinity purified

Stérilité: Sterile filtered

Immunogène: This antibody was purified from whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (114-122) from hemagglutinin influenza conjugated to KLH using maleimide. A residue of cysteine was added to the carboxy terminal end to facilitate coupling.

Type of Immunogen: Purified protein

Isotype: IgG

Information d'application

Indications d'application: Approved: ChrIP, ELISA, IHC, IHC-P (1:500 - 1:2000), IP, WB (1:2000 - 1:10000)

Usage: Anti-HA is optimally suited for monitoring the expression of HA-tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope. The antibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targeted proteins, as expressed in many commonly used expression vectors. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and HA containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide, sterile filtered

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: avoid freeze thaw cycles. Store undiluted.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Détails sur HA-Tag

Antigène: HA-Tag

Autre désignation: Hemagglutinin / HA Tag

Classe de substances: Tag

Sujet: Name/Gene ID: HA Tag

Synonyms: HA Tag