IL-13 anticorps
Aperçu rapide pour IL-13 anticorps (ABIN1102453)
Antigène
Voir toutes IL-13 (IL13) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Marque
- IHC-plus™
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Specificité
- Human IL-13
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Purification
- Immunoaffinity purified
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Immunogène
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E.coli derived recombinant Human IL-13.
Type of Immunogen: Cell extract -
Isotype
- IgG
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Indications d'application
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Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (0.12 - 0.15 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: LS-B7417 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-13 (2 ng/mL), a concentration of 0.12-0.15 μg/mL of this antibody is required. ELISA: To detect hIL-13 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-13 (LS-C104340) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-13. Western Blot: To detect hIL-13 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-13 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. -
Commentaires
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Sterile water
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Concentration
- Lot specific
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Buffer
- Lyophilized from PBS, pH 7.2
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Conseil sur la manipulation
- Avoid freeze/thaw cycles.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- IL-13 (IL13) (Interleukin 13 (IL13))
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Autre désignation
- IL13
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Sujet
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Name/Gene ID: IL13
Family: Hormone
Synonyms: IL13, ALRH, BHR1, Interleukin-13, NC30, p600, IL-13, Interleukin 13 -
ID gène
- 3596
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UniProt
- P35225
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Pathways
- Signalistation JAK/STAT, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport
Antigène
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