CD209b Antigen (CD209B) anticorps

N° du produit ABIN1106055

Détail du produit

Antigène: CD209b Antigen (CD209B)

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: This antibody recognises SIGN-R1, a C-type lectin that has been identified as a murine homologue of the human DC-SIGN related molecule, L-SIGN. Mouse SIGN-R1 is specifically expressed on marginal zone macrophages in the spleen, medullary and subcapsular sinus macrophages in the lymph nodes, and at lower levels on liver sinusoidal endothelial cells. SIGN-R1 has not been detected on murine dendritic cell populations. Like many C-type lectins, SIGN-R1 functions as a pathogen recognition receptor in vivo and plays an important role in defence against pathogens. Antibodies, produced by clone ER-TR9, are reported to block the uptake of neutral polysaccharides by macrophages.

Purification: Ammonium sulphate precipitation

niveau d'endotoxine: Low Endotoxin

Immunogène: Isolated C3H thymic stromal cells. - Spleen cells from immunised rats were fused with cells of the Mouse P3-X63-Ag8.563 myeloma cell line.

Clone: ER-TR9

Isotype: IgM

Information d'application

Indications d'application: Flow Cytometry: 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections: 1/25 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline pH 7.4

Stock: -20 °C

Stockage commentaire: Store the antibody at -20 °C only.

Référence

Weisel, Appelt, Schneider, Horlitz, van Rooijen, Korner, Mach, Winkler: "Unique requirements for reactivation of virus-specific memory B lymphocytes." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 7, pp. 4011-21, (2010) (PubMed).

Détail du antigène

Antigène: CD209b Antigen (CD209B)

Autre désignation: CD209b / DC-SIGNR1 (CD209B Produits)

Synonymes: anticorps 1810030I22Rik, anticorps OtB7, anticorps SIGNR1, anticorps mSIGNR1, anticorps CD209b antigen, anticorps Cd209b

Sujet: SIGN R1 is a specific marker for the identification of macrophage subpopulations present in the marginal zone of spleen (the so-called marginal zone macrophages (MZM)), in the lymph node medulla, and in some strains, in the peritoneal cavity. MZM of the spleen are involved in the clearance of polysaccharides. Mouse SIGN R1 is a C type lectin, like DC SIGN which is expressed on Human dendritic cells (DCs). However, Mmouse SIGN R1 itself is not expressed on DCs. SIGN R1 exists in an aggregated form, resistant to dissociation into monomers upon boiling in SDS under reducing conditions. SIGN R1 mediates the uptake of encapsulated organisms and may be an important mediator for the uptake of microbes in both spleen and lymph node, particularly through the recognition of microbial polysaccharides.Synonyms: CD209 antigen-like protein B, DC-SIGN-related protein 1, OtB7

ID gène: 69165

NCBI Accession: NP_001032889

UniProt: Q8CJ91