TdT anticorps (C-Term)

N° du produit ABIN1107004

Cet anticorps Lapin Polyclonal détecte spécifiquement TdT dans WB. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour TdT anticorps (C-Term) (ABIN1107004)

Antigène: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TdT est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-TdT anticorps

Épitope: C-Term

Specificité: This antibody detects DNTT / TDT at C-term.

Réactivité croisée (Details): Species reactivity (expected):Rat, mouse
Species reactivity (tested):Human

Purification: Immunogen affinity purified

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminal of human TDT .Remarks: Sequence is identical to the related rat and mouse sequence.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Reconstitution: 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3

Agent conservateur: Sodium azide, Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Détails sur TdT

Antigène: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Autre désignation: DNTT / TDT

Sujet: Terminal Deoxynucleotidyl Transferase, also known as TdT and terminal transferase, is a unique DNA polymerase without template direction catalyzes the addition of deoxyribonucleotides onto the 3-prime-hydroxyl end of DNA primers.1 Its gene is mapped to the region 10q23-q24.2 And TDT Cdna contains an open reading frame of 1,530 basepairs corresponding to a protein containing 510 amino acids.3 TDT may be responsible for inserting nucleotides (N regions) at the V(H)-D and D-J(H) junctions of immunoglobulin genes. The enzyme is present in immature thymocytes, some bone marrow cells,transformed pre-B and pre-T cell lines, and leukemia cells. Additionally, TdT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule. Unlike most DNA polymerases it does not require a template. The preferred substrate of this enzyme is a 3'-overhang, but it can also add nucleotides to blunt or recessed 3' ends. Cobalt is a necessary cofactor.Synonyms: DNA nucleotidylexotransferase, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Terminal transferase

ID gène: 1791

NCBI Accession: NP_001017520

UniProt: P04053

Pathways: Réparation de l'ADN