LY96 anticorps

N° du produit ABIN1108190

Détail du produit anti-LY96 anticorps

Antigène: LY96 (Lymphocyte Antigen 96 (LY96))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LY96 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificité: This antibody reacts with soluble MD-2 (sMD-2) and the sMD-2/Toll-like receptor 4 (TLR4, CD284) complex. It reacts with both the monomeric and the polymeric form of sMD-2.

Aucune reactivité croisée: Souris

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Protein G

Immunogène: Baculovirally expressed His-tagged human MD-2

Clone: 4H1

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % bovine serum albumin

Stock: 4 °C

Stockage commentaire: Store at 2 - 8 °C.

Détails sur LY96

Antigène: LY96 (Lymphocyte Antigen 96 (LY96))

Autre désignation: MD-2 (LY96 Produits)

Synonymes: anticorps MD2, anticorps ESOP-1, anticorps MD-2, anticorps ly-96, anticorps Ly-96, anticorps Md2, anticorps lymphocyte antigen 96, anticorps LY96, anticorps Ly96

Sujet: TLRs belong to a family of proteins that specifically recognizes and senses microbial products. They are highly conserved throughout evolution and act as innate immune recognition receptors against many pathogens. TLR4 is a functional receptor for gram-negative bacterial lipopolysaccharides (LPS). TLR4 associates with MD-2 which is absolutely required for LPS-induced activation of TLR4. MD-2 exists as a cell surface protein in association with TLR4. It also exists as secreted forms consisting of MD-2 monomer and multimers. Circulating sMD-2 is mainly present as a doublet of ~20 and 25 kD, representing differentially glycosylated forms. Unlike TLR4, sMD-2 binds directly LPS without the need of soluble CD14 (sCD14). However, LPS-MD-2 interactions are increased when LPS is pretreated with CD14. Only monomeric sMD-2 is biologically active and able to associate with TLR4 and LPS. sMD-2 circulates in plasma of healthy individuals as a non-active, polymeric protein. In septic plasma, the total amount of sMD-2 was strongly elevated and contained both sMD-2 polymers and monomers. Soluble MD-2 is proposed to be an important mediator of organ inflammation during sepsis. During experimental human endotoxemia, the monomeric and total sMD-2 content in plasma increased with the kinetics of an acute phase protein. This parallels enhanced TLR4 costimulatory activity. In vitro studies revealed that sMD-2 release appears to be restricted to endothelial and dendritic cells.Synonyms: ESOP-1, ESOP1, LY96, Lymphocyte antigen 96, MD2

ID gène: 23643

NCBI Accession: NP_001182726

UniProt: Q9Y6Y9

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades