TUBB3 anticorps (N-Term) (FITC)

N° du produit ABIN110981

Il existe 3+ publications pour ce produit. L’anticorps anti-TUBB3 Monoclonal Souris est utilisé pour la détection de TUBB3 dans des échantillons de Vicia faba. Il a été validé pour WB, IF, FACS, IHC (p), IHC (fro) et EIA.

Aperçu rapide pour TUBB3 anticorps (N-Term) (FITC) (ABIN110981)

Antigène: TUBB3 (Tubulin, beta 3 (TUBB3))

Reactivité: Vicia faba

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TUBB3 est conjugé à/à la FITC

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: TU-20

Détail du produit anti-TUBB3 anticorps (FITC)

Épitope: N-Term

Specificité: This antibody recognizes the C-terminal peptide sequence ESESQGPK (aa 441-448) of human class III β-tubulin specific for neurones. The antibody is a highly specific marker for neuronal tissue. TU-20 is very useful for the detection of microtubule structures on fixed cells. MAb TU-20 is widely cross-reactive among species (recognized epitope conserved within all species).

Immunogène: Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry on Frozen and Paraffin Sections. Immunocytotochemistry on Fixed and Permeabilized cells. Suggested working dilution is 1/40. The conjugate was also successfully used on FFPE Sections using Confocal Microscopy.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS containing 15 mM Sodium Azide as preservative, approx. pH 7.4

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2-8 °C for one month or at -20 °C for longer.

Références pour anticorps anti-TUBB3 (FITC) (ABIN110981)

Katsetos, Draber, Kavallaris: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." dans: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, (2012) (PubMed).

Theodorou, Dalembert, Heffelfinger, White, Weissman, Corcoran, Snyder: "A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation." dans: Genes & development, Vol. 23, Issue 5, pp. 575-88, (2009) (PubMed).

Dráberová, Del Valle, Gordon, Marková, Smejkalová, Bertrand, de Chadarévian, Agamanolis, Legido, Khalili, Dráber, Katsetos: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." dans: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, (2008) (PubMed).

Détails sur TUBB3

Antigène: TUBB3 (Tubulin, beta 3 (TUBB3))

Autre désignation: TUBB3 / TUBB4

Sujet: The betaIII-tubulin isotype is present dominantly in cells of neuronal origin and it is one of the earliest marker of neuronal differentiation. Class III beta-tubulin, is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The neuron-associated class III beta-tubulin isotype is most abundant in cells of neuronal origin but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.Synonyms: Tubulin beta-3 chain, Tubulin beta-4, Tubulin beta-III

ID gène: 10381

NCBI Accession: NP_001184110

UniProt: Q13509

Pathways: Dynamique des Microtubules, M Phase