Cytokeratin 18 anticorps (FITC)

N° du produit ABIN111182

L’anticorps Souris Monoclonal anti-Cytokeratin 18 a été validé pour IF, FACS, IHC (p) et IHC (fro). Il convient pour détecter Cytokeratin 18 dans des échantillons de Humain, Souris, Rat, Chien, Porc, Boeuf (Vache), Hamster, Mouton et Truite. Il y a 10+ publications disponibles.

Aperçu rapide pour Cytokeratin 18 anticorps (FITC) (ABIN111182)

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivité: Humain, Souris, Rat, Chien, Porc, Boeuf (Vache), Hamster, Mouton, Truite

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytokeratin 18 est conjugé à/à la FITC

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: Ks18-04

Détail du produit anti-Cytokeratin 18 anticorps (FITC)

Specificité: Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors Specifically Detected: all adenocarcinoma, mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide Reacting: Mr 45 000 polypeptide (human keratin K18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia. Reacts with HeLa and MCF-7 (cell lines tested so far).

Purification: Protein A Chromatography

Immunogène: Human keratin K18 from a HeLa cytoskeletal preparation

Isotype: IgG1

Information d'application

Indications d'application: Immunoflourescence. Cytological material. Flow Cytometry: 1/5-1/10. Immunohistochemistry of Frozen Sections. Immunohistochemistry of Paraffin Sections: 1/10. Working dilution: 1/10 for immunohistochemistry. Incubation Time: 1 h at 37 °C.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-Cytokeratin 18 (FITC) (ABIN111182)

Trowe, Airik, Weiss, Farin, Foik, Bettenhausen, Schuster-Gossler, Taketo, Kispert: "Canonical Wnt signaling regulates smooth muscle precursor development in the mouse ureter." dans: Development (Cambridge, England), Vol. 139, Issue 17, pp. 3099-108, (2012) (PubMed).

Hantel, Lewrick, Schneider, Zwermann, Perren, Reincke, Süss, Beuschlein: "Anti insulin-like growth factor I receptor immunoliposomes: a single formulation combining two anticancer treatments with enhanced therapeutic efficiency." dans: The Journal of clinical endocrinology and metabolism, Vol. 95, Issue 2, pp. 943-52, (2010) (PubMed).

Sharma, Cantz, Richter, Eckert, Henschler, Wilkens, Jochheim-Richter, Arseniev, Ott: "Human cord blood stem cells generate human cytokeratin 18-negative hepatocyte-like cells in injured mouse liver." dans: The American journal of pathology, Vol. 167, Issue 2, pp. 555-64, (2005) (PubMed).

Ikenouchi, Matsuda, Furuse, Tsukita: "Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail." dans: Journal of cell science, Vol. 116, Issue Pt 10, pp. 1959-67, (2003) (PubMed).

Stumptner, Fuchsbichler, Heid, Zatloukal, Denk: "Mallory body--a disease-associated type of sequestosome." dans: Hepatology (Baltimore, Md.), Vol. 35, Issue 5, pp. 1053-62, (2002) (PubMed).

Bantel, Ruck, Gregor, Schulze-Osthoff: "Detection of elevated caspase activation and early apoptosis in liver diseases." dans: European journal of cell biology, Vol. 80, Issue 3, pp. 230-9, (2001) (PubMed).

Gomi, Yokoyama, Fujimoto, Ikeda, Katoh, Itoh, Itohara: "Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions." dans: Neuron, Vol. 14, Issue 1, pp. 29-41, (1995) (PubMed).

Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." dans: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, (1991) (PubMed).

Lauerová, Kovarik, Bártek, Rejthar, Vojt?sek: "Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule." dans: Hybridoma, Vol. 7, Issue 5, pp. 495-504, (1989) (PubMed).

Moll, Franke, Schiller, Geiger, Krepler: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." dans: Cell, Vol. 31, Issue 1, pp. 11-24, (1983) (PubMed).

Détails sur Cytokeratin 18

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Autre désignation: Cytokeratin 18

Sujet: Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18

ID gène: 3875

NCBI Accession: NP_000215

UniProt: P05783

Pathways: Apoptose, Caspase Cascade in Apoptosis