Transferrin Receptor anticorps (FITC)

N° du produit ABIN1112226

Cet anticorps anti-Transferrin Receptor est un anticorps Souris Monoclonal détectant Transferrin Receptor dans FACS et IF. Adapté pour Humain.

Aperçu rapide pour Transferrin Receptor anticorps (FITC) (ABIN1112226)

Antigène: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Transferrin Receptor est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: FG2-12

Détail du produit anti-Transferrin Receptor anticorps (FITC)

Attributs du produit: Monoclonal Mouse Anti-Human CD71.

Isotype: IgG1

Information d'application

Indications d'application: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

Commentaires:

Fluorescein isothiocyanate (Molecular Probes).

Prélèvement de l'échantillon: 1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD71 FITC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive FITC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 100 µl of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS ( It better that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis. MDS (Myelodysplastic Syndrome) Sample from a Human Donor.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur Transferrin Receptor

Antigène: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Autre désignation: CD71

Sujet: Human Transferrin Receptor which is expressed on proliferating lymphoblasts, majority human tumour cell lines, normal peripheral blood monocytes. The CD71 antibody in routine is used along with the glycophorin A to develop a flow-cytometric approach to the evaluation of erythroid and myeloid dysplasia in patients with Myelodysplatic Syndromes (MDS)1. Another of the applications of the CD71 antibody, is the recount of retyculocites2, immature red corpuscles, without nucleus, that contain ribonucleic acid (RNA). peripheral blood, they delay approximately a day in losing their RNA and to be become erythrocytes. Its recount provides information on the production of erythrocytes.

Pathways: Transition Metal Ion Homeostasis