His Tag anticorps

N° du produit ABIN1112988

Cet anticorps anti-His Tag est un anticorps Souris détectant His Tag dans WB, IP et IF. Adapté pour .

Aperçu rapide pour His Tag anticorps (ABIN1112988)

Antigène: His Tag

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Conjugué: Cet anticorp His Tag est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Détail du produit anti-His Tag anticorps

Séquence: HHHHHH

Specificité: Detect over-expressed or recombinant proteins containing the 6 × His epitope tag.

Immunogène: A synthetic peptide 6 × His conjugated to KLH.

Isotype: IgG1

Information d'application

Indications d'application: WB (1:5000), IP (1:100), IF (1:2000). Other applications have not been tested. The optimal dilutions should be determined by end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Agent conservateur: Sodium azide, Thimerosal (Merthiolate)

Stock: -20 °C

Stockage commentaire: Store at -20 °C for 1 year. Or aliquot and store at -80 °C for long term. Avoid repeated freeze and thaw cycles.

Date de péremption: 12 months

Détails sur His Tag

Antigène: His Tag

Classe de substances: Tag

Sujet: A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems. Affinity purification using a polyhistidine-tag usually results in relatively pure protein when the recombinant protein is expressed in prokaryotic organisms. Depending on downstream applications including the purification of protein complexes to study protein interactions, purification from higher organisms such as yeasts or other eukaryotes may require a tandem affinity purification using two tags to yield higher purity. The most common polyhistidine tags are formed of six histidine (6xHis tag) residues - preceded by Methionine - which are added at the C-terminal or N-terminal of the protein of interest.