beta Actin anticorps

N° du produit ABIN1113002

L’anticorps Souris Monoclonal anti-beta Actin a été validé pour WB, IHC, IF et IP. Il convient pour détecter beta Actin dans des échantillons de Souris, Rat, Poulet, Lapin, Chien, Porc, Boeuf (Vache), Mouton, Cobaye, Cat, Sangsue et Carpe.

Aperçu rapide pour beta Actin anticorps (ABIN1113002)

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivité: Souris, Rat, Poulet, Lapin, Chien, Porc, Boeuf (Vache), Mouton, Cobaye, Cat, Sangsue, Carpe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp beta Actin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Détail du produit anti-beta Actin anticorps

Specificité: Detect the epitope located on N-terminal of beta-Actin.

Immunogène: Gizzard Actin of chicken origin.

Isotype: IgG1

Information d'application

Indications d'application: Western Blot (1:100-1000), IF (1:50-500) ,IHC (1:50-500) . Other applications have not been tested. The optimal dilutions should be determined by end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Agent conservateur: Sodium azide, Thimerosal (Merthiolate)

Stock: -20 °C

Stockage commentaire: Store at -20 °C for 1 year. Avoid repeated freeze and thaw cycles.

Date de péremption: 12 months

Détails sur beta Actin

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Autre désignation: beta-Actin

Sujet: Beta-actin (gene name ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Karakozova et al. (2006) found that approximately 40% of intracellular beta-actin is arginylated in vivo. In both wildtype and Ate1-null cells beta-actin was stable, suggesting that arginylation does not induce beta-actin degradation. And arginylation of beta-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo. 1 Nitric oxide (NO) is a paracrine mediator of vascular and platelet function that is produced in the vasculature by NO synthase-3. And beta-actin plays a critical role in regulating NO formation and signaling in platelets. 2 Beta actin is also usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western Blotting.

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction