CD68 anticorps

N° du produit ABIN111830

Détail du produit anti-CD68 anticorps

Antigène: CD68 (CD68 Molecule (CD68))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD68 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Specificité: CD68 Antibody (Cat#BM4000) recognises a single chain glycoprotein of 110 kDa that is expressed predominantly on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes.

Réactivité croisée (Details): Species reactivity (tested):Rat.

Purification: Affinity Chromatography on Protein G

Immunogène: Rat spleen cells. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.

Clone: ED1

Isotype: IgG1

Information d'application

Indications d'application: RIA. Western blotting. Immunoprecipitation. Flow Cytometry: Use 10 μL of 1/50-1/100 diluted CD68 antibody to label 10^6 cells in 100 μL(Results may be enhanced using membrane permeabilisation). Immunohistology on Frozen Sections. Immunohistology on Paraffin Sections (1/100): Requires protein digestion pretreatment ofParaffin sections with Pronase or Trypsin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-CD68 (ABIN111830)

Meyer, Cunea, Sonntag-Bensch, Welker, Licha, Holz, Schmitz-Valckenberg: "In vivo imaging of a new indocyanine green micelle formulation in an animal model of laser-induced choroidal neovascularization." dans: Investigative ophthalmology & visual science, Vol. 55, Issue 10, pp. 6204-12, (2014) (PubMed).

Schneider, Meusel, Renker, Bauer, Holzinger, Roeder, Wanner, Gekle, Sauvant: "Low-dose indomethacin after ischemic acute kidney injury prevents downregulation of Oat1/3 and improves renal outcome." dans: American journal of physiology. Renal physiology, Vol. 297, Issue 6, pp. F1614-21, (2009) (PubMed).

Détails sur CD68

Antigène: CD68 (CD68 Molecule (CD68))

Autre désignation: CD68 (CD68 Produits)

Synonymes: anticorps Lamp4, anticorps Scard1, anticorps gp110, anticorps GP110, anticorps LAMP4, anticorps SCARD1, anticorps CD68 molecule, anticorps CD68 antigen, anticorps Cd68 molecule, anticorps CD68, anticorps Cd68

Sujet: The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.Synonyms: Gp110, Macrophage marker, Macrosialin

ID gène: 287435

NCBI Accession: NP_001026808

UniProt: Q4FZY1