S100A8 anticorps (Biotin)

N° du produit ABIN111891

Détail du produit anti-S100A8 anticorps (Biotin)

Antigène: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp S100A8 est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificité: This Monoclonal antibody 8-5C2 detects a very distinct epitope in the central part of the molecule, in between the two Ca2+-binding domains (different from the S 13.67 epitope). This antibody identifies the Ca2+-binding light subunit of the inflammatory L-1 protein complex, also called S100A8 or Calgranulin A. It is useful for the identification of the 8 kDa subunit in various immunological techniques. Histological and serological data indicate that MRP8 is associated with chronic stages of inflammatory diseases. Antigen Distribution Isolated Cells: The antigen is found in granulocytes and monocytes but not in other blood cells. In cultured monocytes, maximum MRP8 is expressed after 3-4 days. Myeloid leukemia stain positive. Tissue Sections: In tissues MRP8 is found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages synthesise MRP8 increasingly during the late stages of inflammation. A low MRP8 (and high MRP-14) expression by macrophages was also reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases such as chronic rheumatoid arthritis or chronic rejection after allograft transplantation, MRP8 and MRP14 positive cells consist of different subpopulations. The antibody reacts with Bovine spleen but does not react with Rat tissues. It shows a diffuse reaction with Swine spleen.

Réactivité croisée (Details): Species reactivity (tested):Human: MRP8 in stimulated monocytes and macrophages in late phase or chronic inflammation.

Purification: Protein A Chromatography

Immunogène: Cultured Human monocytes. Remarks: The antigen is MRP8, the epitope is suspected in the central portion of the peptide.

Clone: 8-5C2

Isotype: IgG1

Information d'application

Indications d'application: ELISA. Flow Cytometry. Immunohistochemistry (IHC) on: Frozen Sections: 2 μg/mL (1/100). Paraffin Sections: 5-10 μg/mL (1/20-1/50). No pretreatment for antigen retrieval necessary,microwave treatment in 0.01M citrate pH 6.0 may enhance the reactivity. Suggested positive control: Human tonsil. Has been described to work Dot blots.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water (This stock solution contains 0.2 mg/mL IgG).

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2, 0.05 % (v/v) Kathon, 5 mg/mL BSA

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Dilute only prior to immediate use

Stock: 4 °C

Stockage commentaire: Store the antibody at 2-8 °C before opening. DO NOT FREEZE! This product is stable at 2-8 °C as an undiluted liquid. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Références pour anticorps anti-S100A8 (Biotin) (ABIN111891)

Wong, Zhou, Li, Tong, Zhao, Li, Yu, Koh, Beuerman: "Proteomic profiling of inflammatory signaling molecules in the tears of patients on chronic glaucoma medication." dans: Investigative ophthalmology & visual science, Vol. 52, Issue 10, pp. 7385-91, (2011) (PubMed).

Mortezai, Harder, Schnabel, Moors, Gauly, Schlüter, Wagener, Buck: "Tandem affinity depletion: a combination of affinity fractionation and immunoaffinity depletion allows the detection of low-abundance components in the complex proteomes of body fluids." dans: Journal of proteome research, Vol. 9, Issue 12, pp. 6126-34, (2010) (PubMed).

Soupene, Serikov, Kuypers: "Characterization of an acyl-coenzyme A binding protein predominantly expressed in human primitive progenitor cells." dans: Journal of lipid research, Vol. 49, Issue 5, pp. 1103-12, (2008) (PubMed).

Rosenberger, Thorey, Werner, Boukamp: "A novel regulator of telomerase. S100A8 mediates differentiation-dependent and calcium-induced inhibition of telomerase activity in the human epidermal keratinocyte line HaCaT." dans: The Journal of biological chemistry, Vol. 282, Issue 9, pp. 6126-35, (2007) (PubMed).

Zaba, Cardinale, Gilleaudeau, Sullivan-Whalen, Suárez-Fariñas, Suárez Fariñas, Fuentes-Duculan, Novitskaya, Khatcherian, Bluth, Lowes, Krueger: "Amelioration of epidermal hyperplasia by TNF inhibition is associated with reduced Th17 responses." dans: The Journal of experimental medicine, Vol. 204, Issue 13, pp. 3183-94, (2007) (PubMed).

Pedersen, Oster, Bundgaard, Höllsberg: "Induction of cell-cell fusion from without by human herpesvirus 6B." dans: Journal of virology, Vol. 80, Issue 19, pp. 9916-20, (2006) (PubMed).

Lukás, Dráber, Bucek, Dráberová, Viklický, Dolezel: "Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord." dans: Histochemistry, Vol. 100, Issue 6, pp. 495-502, (1994) (PubMed).

Bártek, Bártková, Vojt?sek, Stasková, Lukás, Rejthar, Kovarík, Midgley, Gannon, Lane: "Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of human malignancies." dans: Oncogene, Vol. 6, Issue 9, pp. 1699-703, (1991) (PubMed).

Détails sur S100A8

Antigène: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Autre désignation: S100A8 / Calgranulin-A / MRP8 (S100A8 Produits)

Synonymes: anticorps 60B8AG, anticorps CAGA, anticorps CFAG, anticorps CGLA, anticorps CP-10, anticorps L1Ag, anticorps MA387, anticorps MIF, anticorps MRP8, anticorps NIF, anticorps P8, anticorps Mrp8, anticorps S100A8, anticorps 60B8Ag, anticorps AI323541, anticorps B8Ag, anticorps CFAg, anticorps Caga, anticorps p8, anticorps MRP-8, anticorps S100 calcium binding protein A8, anticorps S100 calcium binding protein A8 (calgranulin A), anticorps S100A8, anticorps S100a8

Sujet: Synonyms: CAGA, CFAG, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, MRP-8, Migration inhibitory factor-related protein 8, S100 calcium-binding protein A8, S100-A8, Urinary stone protein band A

ID gène: 9606

UniProt: P05109

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins