S100A8 anticorps (C-Term)

N° du produit ABIN111892

Cet anticorps Souris Monoclonal détecte spécifiquement S100A8 dans FACS, IHC (p), EIA, IHC (fro) et DB. Il présente une réactivité envers Humain, Rat et Porc.

Aperçu rapide pour S100A8 anticorps (C-Term) (ABIN111892)

Antigène: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reactivité: Humain, Rat, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp S100A8 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB)

Clone: S13-67

Détail du produit anti-S100A8 anticorps

Épitope: C-Term

Specificité: Clone S13.67 identifies MRP8: The antibody is useful in various immunological techniques. Histological and serological data indicate that MRP8 is associated with chronic stages of inflammatory diseases. This clone also stains cells in rat spleen, indiating significant cross reactivity with the corresponding Rat MRP8. Antigen Distribution on Isolated Cells: The antigen is found in granulocytes and monocytes. It is absent from other blood cells. In cultured monocytes, maximum MRP8 is expressed after 3-4 days. Antigen Distribution on Tissue Sections: In the tissue, MRP-8 is only found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages increasingly synthesise MRP-8 during the late stages of inflammation. A low MRP-8 (and high MRP-14) expression by macrophages was also reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases such as chronic rheumatoid arthritis MRP8 and MRP14 positive cells consist of different subpopulations. During early inflammation endothelial cells are also positive with MRP8/MRP14.

Purification: Affinity Chromatography.

Immunogène: Cultured Human monocytes. Remarks: Antigen: MRP8. Epitope: Suspected in the N- or C-terminal Domain.

Isotype: IgG1

Information d'application

Indications d'application: ELISA. Immunohistochemistry on Frozen Sections: 1-2 μg/mL (1/100-1/200). Immunohistochemistry on Paraffin Sections: 4 μg/mL (1/50). Pre-treatment for antigenretrieval not required. Has been described to work in FACS and Dot blots. Suggested Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore by adding 0.5 mL distilled water.

Concentration: 0.2 mg/mL

Buffer: Stock solution contains PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer and 0.05 % (v/v) Kathon CG as preservative.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: Do Not Use Sodium Azide as preservative.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Détails sur S100A8

Antigène: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Autre désignation: S100A8 / Calgranulin-A / MRP8

Sujet: MRP8 is the Ca2+-binding light subunit of Calprotectin. MRP8 forms Ca2+-dependent or complexes with MRP14 (S100A9, Calgranulin B). It also forms disulfide-linked homodimers under the influence of hypochlorite, a process thought to abrogate the chemotactic property of MRP8.Synonyms: CAGA, CFAG, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, MRP-8, Migration inhibitory factor-related protein 8, S100 calcium-binding protein A8, S100-A8, Urinary stone protein band A

ID gène: 9606

UniProt: P05109

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins