CD31 anticorps (Biotin)

N° du produit ABIN111979

Détail du produit anti-CD31 anticorps (Biotin)

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD31 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography.

Immunogène: Mouse macrophage precursor cells. The antigen is a glutaraldehyde (0.05%) and paraformaldehyde (1%) resistant 140kD surface glycoprotein (reducing and non reducing conditions). The antigen expression closely follows that of CD34.

Clone: ER-MP12

Isotype: IgG2a

Information d'application

Indications d'application: Immunohistochemistry on acetone fixed Frozen Sections (1/1600 = 0.4 μg/mL)and Formalin Fixed Paraffin Sections (1/40 = 15 μg/mL). Antigen Retrieval: Proteinase K. Suggested Positive Control: Mouse spleen. Has been described to work in FACS analysis: 4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water (0.6 mg/mL stock solution).

Concentration: 0.6 mg/mL

Buffer: PBS, pH 7.2 containing 5 mg/mL BSA as a stabilizer and 0.05 % Kathon as a preservative.

Agent conservateur: Kathon CG

Précaution d'utilisation: This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store (in aliquots) at -20 °C.

Références pour anticorps anti-CD31 (Biotin) (ABIN111979)

Mateos, Landeira-Abia, Fafián-Labora, Fernández-Pernas, Lesende-Rodríguez, Fernández-Puente, Fernández-Moreno, Delmiro, Martín, Blanco, Arufe: "iTRAQ-based analysis of progerin expression reveals mitochondrial dysfunction, reactive oxygen species accumulation and altered proteostasis." dans: Stem cell research & therapy, Vol. 6, pp. 119, (2016) (PubMed).

Morikawa, Kajimura, Nakamura, Hishiki, Nakanishi, Yukutake, Nagahata, Ishikawa, Hattori, Takenouchi, Takahashi, Ishii, Matsubara, Kabe, Uchiyama, Nagata, Gadalla, Snyder, Suematsu: "Hypoxic regulation of the cerebral microcirculation is mediated by a carbon monoxide-sensitive hydrogen sulfide pathway." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 4, pp. 1293-8, (2012) (PubMed).

Sakamoto, Geisel, Kim, Wyatt, Sellers, Smiley, Cooper, Russell, Rhoades: "Fibrinogen regulates the cytotoxicity of mycobacterial trehalose dimycolate but is not required for cell recruitment, cytokine response, or control of mycobacterial infection." dans: Infection and immunity, Vol. 78, Issue 3, pp. 1004-11, (2010) (PubMed).

Larghero, Venè, Minghelli, Travaini, Morini, Ferrari, Pfeffer, Noonan, Albini, Benelli: "Biological assays and genomic analysis reveal lipoic acid modulation of endothelial cell behavior and gene expression." dans: Carcinogenesis, Vol. 28, Issue 5, pp. 1008-20, (2007) (PubMed).

Li, Sainson, Shi, Leek, Harrington, Preusser, Biswas, Turley, Heikamp, Hainfellner, Harris: "Delta-like 4 Notch ligand regulates tumor angiogenesis, improves tumor vascular function, and promotes tumor growth in vivo." dans: Cancer research, Vol. 67, Issue 23, pp. 11244-53, (2007) (PubMed).

Burbaeva, Boksha, Tereshkina, Savushkina, Starodubtseva, Turishcheva, Mukaetova-Ladinska: "Systemic neurochemical alterations in schizophrenic brain: glutamate metabolism in focus." dans: Neurochemical research, Vol. 32, Issue 9, pp. 1434-44, (2007) (PubMed).

Li, Otsu, Murphy, Raymond: "Developmental decrease in NMDA receptor desensitization associated with shift to synapse and interaction with postsynaptic density-95." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 23, Issue 35, pp. 11244-54, (2003) (PubMed).

Détails sur CD31

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Autre désignation: CD31 / PECAM1 (PECAM1 Produits)

Synonymes: anticorps CD31, anticorps CD31/EndoCAM, anticorps GPIIA', anticorps PECA1, anticorps PECAM-1, anticorps endoCAM, anticorps C85791, anticorps Cd31, anticorps Pecam, anticorps platelet/endothelial cell adhesion molecule 1, anticorps platelet and endothelial cell adhesion molecule 1, anticorps pecam1, anticorps PECAM1, anticorps Pecam1

Sujet: Anti-PECAM-1 is a general marker for the detection of a major population of haematopoietic stem cells and is useful for the accumulation of early murine macrophage precursors from bone marrow cell suspensions by FACS. An even more precise characterization of such isolated and concentrated precursor cells is achieved when ER-MP12 is combined with other markers like ER-MP20 (anti Ly-6C) or ER-MP58. The ER-MP12 antigen is also expressed by endothelial cells.Synonyms: End °CAM, GPIIA', PECAM-1, Platelet endothelial cell adhesion molecule

ID gène: 10090

UniProt: Q08481

Pathways: Regulation of Actin Filament Polymerization