Transferrin Receptor anticorps (Biotin)

N° du produit ABIN111981

Cet anticorps anti-Transferrin Receptor est un anticorps Rat Monoclonal détectant Transferrin Receptor dans FACS et IHC (fro). Adapté pour Souris.

Aperçu rapide pour Transferrin Receptor anticorps (Biotin) (ABIN111981)

Antigène: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Transferrin Receptor est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: ER-MP21

Détail du produit anti-Transferrin Receptor anticorps (Biotin)

Specificité: ER-MP21 stains proliferating cells of all types as well as cells taking up iron for special needs, such as late erythroid precursors and some mature macrophages. The antibody inhibits the iron uptake and the proliferation of macrophage precursors from bone marrow and early macrophage precursor cell lines. ER-MP21 recognizes the transferrin receptor but does not compete with transferrin binding. The antigen is a 200 kDa protein (non-reducing conditions) consisting of two identical chains. The recognized epitope of the receptor is not located at the transferrin binding site. Antigen Distribution: Macrophage progeny at day 7 in bone marrow cultures, treated in parallel on various days by irradiation (1500 rad 60Co γ-radiation) or addition of ER-MP21 (30 μg/mL final concentration). Cell numbers were quantified using the MTT assay and expressed relative to untreated controls. Relative growth inhibition calculated from data represented in left figure. For mAbor radiation treated cultures, the number of cell cycles needed to reach the cell number in untreated controls was calculated. Values obtained for irradiated cultures were taken as 100 % inhibition controls and values for ER-MP21 - treated cultures were expressed relative to these (See Figure. 1).

Réactivité croisée (Details): Species reactivity (tested):Mouse: Transferrin Receptor.

Purification: Affinity Chromatography

Immunogène: Macrophages precursor cells.

Isotype: IgG2a

Information d'application

Indications d'application: Flow Cytometry (5 μg): Use 5 μL of the suggested dilution to label 10^6 cells. Immunohistochemistry on Frozen Sections: 2 μg/mL (1/200). Does not react on routinely processed Paraffin Sections. Suggested positive control: Mouse spleen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.4 mg/mL

Buffer: Stock solution contains PBS, pH 7.2 with 0.09 % Sodium Azide as preservative and 10 mg/mL BSA as stabilizer

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Détails sur Transferrin Receptor

Antigène: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Autre désignation: CD71 / TFRC

Sujet: The transferrin receptor has been structurally characterized as a sulfide bound dimer of identical glycoprotein subunits of 95 kDa. The transferrin receptor is not present on resting blood lymphocytes. On PBL, the receptor appears after activation. The expression of transferrin receptor is coordinately regulated with cell growth. Present on T and B cell lines. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein, it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.Synonyms: TfR1, Transferrin receptor protein 1, p90

ID gène: 22042

NCBI Accession: NP_035768

UniProt: Q62351

Pathways: Transition Metal Ion Homeostasis