AIMP1 anticorps

N° du produit ABIN112011

Cet anticorps anti-AIMP1 est un anticorps Souris Monoclonal détectant AIMP1 dans WB, EIA, IHC (p) et IHC (fro). Adapté pour Humain et Rat.

Aperçu rapide pour AIMP1 anticorps (ABIN112011)

Antigène: AIMP1 (Aminoacyl tRNA Synthetase Complex-Interacting Multifunctional Protein 1 (AIMP1))

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp AIMP1 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 546-2

Détail du produit anti-AIMP1 anticorps

Specificité: This antibody reacts with Endothelial-Monocyte-Activating Polypeptide II (EMAP II).

Réactivité croisée (Details): Species reactivity (tested):Human, Rat.

Purification: Affinity Chromatography.

Immunogène: Recombinant Human EMAPII. The epitope has not been further characterized.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry on Frozen Sections: 10 μg/mL (1/40)Immunohistochemistry on Paraffin Sections: 10 μg/mL (1/40). Microwave pretreatment forantigen retrieval is recommended. Recommended Positive Control: Rat spleen. Has been described to work in ELISA and Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Reconstitute by adding 0.5 mL distilled water.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur AIMP1

Antigène: AIMP1 (Aminoacyl tRNA Synthetase Complex-Interacting Multifunctional Protein 1 (AIMP1))

Autre désignation: SCYE1 / EMAP2

Sujet: EMAPII is a proinflammatory cytokine and chemoattractant of macrophages. The 20 kDa mature peptide binds to endothelial cells and leukocytes like macrophages. EMAP II activates endothelial cells, by elevation of cytosolic free calcium concentration, release of von Willebrand factor, induction of tissue factor, and expression of adhesion molecules E-selectin and P-selectin. It further activates resting monocytes by elevating cytosolic free calcium concentration, inducing TNFalpha and tissue factor and stimulating chemotaxis. EMAP II has been shown in a tumor model to sensitize the local tumor vasculature to the action of TNFalpha, rendering resistant tumors sensitive to immunotherapy by TNFalpha.Synonyms: AIMP1, Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, EMAP-2, EMAP-II, Endothelial monocyte-activating polypeptide 2, Endothelial monocyte-activating polypeptide II, Multisynthase complex auxiliary component p43, Small inducible cytokine subfamily E member 1

ID gène: 9255

NCBI Accession: NP_001135887

UniProt: Q12904