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CTXB anticorps (HRP)

L’anticorps Lapin Polyclonal anti-CTXB a été validé pour EIA, WB et IHC (fro). Il convient pour détecter CTXB dans des échantillons de Vibrio cholerae.
N° du produit ABIN112703

Aperçu rapide pour CTXB anticorps (HRP) (ABIN112703)

Antigène

CTXB (CtxB Gene Product (CTXB))

Reactivité

  • 7
  • 1
Vibrio cholerae

Hôte

  • 5
  • 2
  • 1
Lapin

Clonalité

  • 5
  • 3
Polyclonal

Conjugué

  • 5
  • 1
  • 1
  • 1
Cet anticorp CTXB est conjugé à/à la HRP

Application

  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Enzyme Immunoassay (EIA), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Specificité

    This antibody detects the beta subunit of Cholera toxin.

    Immunogène

    Purified Choleragenoid.
  • Indications d'application

    Immunohistochemistry on frozen sections (1: 20 - 1: 200). ELISA (1: 200 - 1: 1000). Western blot (1: 20 - 1: 200).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1-2 mg/mL

    Buffer

    Phosphate buffered saline, pH 7.2, containing 0.002 % Thimerosal and 1 % Bovine serum albumin

    Agent conservateur

    Thimerosal (Merthiolate)

    Précaution d'utilisation

    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Antigène

    CTXB (CtxB Gene Product (CTXB))

    Autre désignation

    Cholera Enterotoxin Subunit B

    Sujet

    The Beta subunit of cholera toxin binds to a GM1-ganglioside receptor which is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin Alpha subunit into cells. The holotoxin consists of a pentameric ring of Beta subunits whose central pore is occupied by the Alpha subunit. The Alpha subunit contains two chains, A1 and A2, linked by a disulfide bridge. The Alpha subunit (and Cholera toxin) activate the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.Synonyms: Cholera enterotoxin B chain, Cholera enterotoxin gamma chain, Choleragenoid, VC_1456, ctxB, toxB

    ID gène

    2613962

    NCBI Accession

    NP_231099

    UniProt

    P01556
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