APOA1 anticorps (HRP)

N° du produit ABIN114061

Cet anticorps Mouton Polyclonal détecte spécifiquement APOA1 dans WB et EIA. Il présente une réactivité envers Humain.

Aperçu rapide pour APOA1 anticorps (HRP) (ABIN114061)

Antigène: APOA1 (Apolipoprotein A-I (APOA1))

Reactivité: Humain

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp APOA1 est conjugé à/à la HRP

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-APOA1 anticorps (HRP)

Specificité: This antibody recognizes Apo-A1 and HDL. It does not react with other plasma proteins, including albumin or immunoglobulin fractions. Does not cross react with apolipoprotein B, LDL and VLDL.

Purification: Affinity chromatography

Immunogène: Human Apolipoprotein A1

Information d'application

Indications d'application: ELISA (1/5,000 - 1/20,000). Can be used in a two site assay with BM785 bound to themicrotitre plate and ABIN114061 for detection. Higher titers can be used when TMB is usedas the substrate). Western blot (1/100 -1/600).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL (OD280)

Buffer: PBS containing 50 % glycerol, 0.5 % BSA and 0.01 % Thimerosal.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur APOA1

Antigène: APOA1 (Apolipoprotein A-I (APOA1))

Autre désignation: Apolipoprotein A I (APO AI)

Sujet: Apolipoprotein A I promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids, an 18 amino acid signal peptide is removed co-translationally and a 6 amino acid propeptide is cleaved post-translationally. Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease. The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I. The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.Synonyms: APOA1, Apo-AI, ApoA-I, ApoAI

ID gène: 335

NCBI Accession: NP_000030

UniProt: P02647

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism