Peptide-ELISA: 1/50 - 1/200. In a simple ELISA using 1 g of antigen coated per well, the50 % OD was observed at an antibody dilution of 1/100. With 1 g of peptide-BSA coatedper well the 50 % OD was observed at 1/2500. Under the same conditions the end pointwas observed at 1/80K. Competitive ELISA: pep-BSA plates, coated at 1 g/well, antibodydiluted 1/10K. Pep-BSA displaces in the range 0.1 to 100 μg/mL. The free peptide does notdisplace in this assay system. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
1.8 mg/mL (OD280)
Buffer
PBS, pH 7.2, with 0.09 % sodium azide as preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Antigène
BMP10
(Bone Morphogenetic Protein 10 (BMP10))
Autre désignation
BMP10
Sujet
Bone morphogenetic protein-10 (BMP10) belongs to the transforming growth factor beta (TGFß) family. BMPs, named for their initial biological activity of inducing ectopic bone formation, have been linked to multiple steps of cardiac development. BMP10 is translated as a precursor protein (preproprotein) with N-terminal hydrophobic leader peptide followed by prodomain and the mature C-terminal region that is released after the cleavage at multibasic motif RXRR. Data strongly suggest that BMP10 is an essential component in modulating cardiomyocyte proliferation and maturation during cardiac ventricular development.Synonyms: BMP-10, Bone morphogenetic protein 10