Collagen IV anticorps

N° du produit ABIN114114

Cet anticorps anti-Collagen IV est un anticorps Lapin Polyclonal détectant Collagen IV dans IHC (p), IF et EIA. Adapté pour Souris et Orang-Utan.

Aperçu rapide pour Collagen IV anticorps (ABIN114114)

Antigène: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivité: Souris, Orang-Utan

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Collagen IV est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Détail du produit anti-Collagen IV anticorps

Specificité: Antibody BP975 is specific for Mouse collagen type IV. The following cross reactivities have been observed: Mouse Type IV: 100 % Mouse Type III: < 0.1 % Mouse Type II: < 0.1 % Mouse Type I: < 0.1 % Human Type V: < 0.1 % Human Type IV: < 0.1 % Mouse Fibronectin: < 0.1 % Mouse Laminin: < 0.1 %

Réactivité croisée (Details): Species reactivity (expected):OrangutanSpecies reactivity (tested):Mouse

Purification: Antigen Affinity Chromatography

Immunogène: Collagen IV purified from Mouse EHS tumour

Information d'application

Indications d'application: Immunohistochemistry on Paraffin Embedded Sections (1/250-1/1,000): Antigen retrievalis not necessary but may improve results, the recommended fixatives are AFA, 5-10 % buffered formalin or Bouin's. Immunofluorescence (1/40-1/60). ELISA (1/100-1/400).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store undiluted (in aliquots) at -20 °C.

Détails sur Collagen IV

Antigène: Collagen IV (COL4) (Collagen, Type IV (COL4))

Autre désignation: Collagen Type IV

Sujet: Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas.Synonyms: COL4A1

ID gène: 12826

NCBI Accession: NP_034061

UniProt: P02463